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Specification of hepatopancreas progenitors in zebrafish by hnf1ba and wnt2bb.

http://www.ncbi.nlm.nih.gov/pubmed/23720049

Although the liver and ventral pancreas are thought to arise from a common multipotent progenitor pool, it is unclear whether these progenitors of the hepatopancreas system are specified by a common genetic mechanism. Efforts to determine the role of Hnf1b and Wnt signaling in this crucial process have been confounded by a combination of factors, including a narrow time frame for hepatopancreas specification, functional redundancy among Wnt ligands, and pleiotropic defects caused by either severe loss of Wnt signaling or Hnf1b function. Using a novel hypomorphic hnf1ba zebrafish mutant that exhibits pancreas hypoplasia, as observed in HNF1B monogenic diabetes, we show that hnf1ba plays essential roles in regulating β-cell number and pancreas specification, distinct from its function in regulating pancreas size and liver specification, respectively. By combining Hnf1ba partial loss of function with conditional loss of Wnt signaling, we uncover a crucial developmental window when these pathways synergize to specify the entire ventrally derived hepatopancreas progenitor population. Furthermore, our in vivo genetic studies demonstrate that hnf1ba generates a permissive domain for Wnt signaling activity in the foregut endoderm. Collectively, our findings provide a new model for HNF1B function, yield insight into pancreas and β-cell development, and suggest a new mechanism for hepatopancreatic specification.

Pubmed ID: 23720049 RIS Download

Mesh terms: Animals | Animals, Genetically Modified | Cell Differentiation | Hepatocyte Nuclear Factor 1-beta | Hepatopancreas | Signal Transduction | Stem Cells | Wnt Proteins | Zebrafish | Zebrafish Proteins

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Associated grants

  • Agency: NIDDK NIH HHS, Id: 1DP2DK098092
  • Agency: NIDDK NIH HHS, Id: DK61245
  • Agency: NIDDK NIH HHS, Id: DK75032
  • Agency: NIDDK NIH HHS, Id: DP2 DK098092
  • Agency: NIDDK NIH HHS, Id: P30 DK026743
  • Agency: NIDDK NIH HHS, Id: P30 DK058404
  • Agency: NIDDK NIH HHS, Id: U01 DK089570

ZFIN (Data, Gene Expression)

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