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Crystal structure of human Karyopherin β2 bound to the PY-NLS of Saccharomyces cerevisiae Nab2.

http://www.ncbi.nlm.nih.gov/pubmed/23535894

Import-Karyopherin or Importin proteins bind nuclear localization signals (NLSs) to mediate the import of proteins into the cell nucleus. Karyopherin β2 or Kapβ2, also known as Transportin, is a member of this transporter family responsible for the import of numerous RNA binding proteins. Kapβ2 recognizes a targeting signal termed the PY-NLS that lies within its cargos to target them through the nuclear pore complex. The recognition of PY-NLS by Kapβ2 is conserved throughout eukaryotes. Kap104, the Kapβ2 homolog in Saccharomyces cerevisiae, recognizes PY-NLSs in cargos Nab2, Hrp1, and Tfg2. We have determined the crystal structure of Kapβ2 bound to the PY-NLS of the mRNA processing protein Nab2 at 3.05-Å resolution. A seven-residue segment of the PY-NLS of Nab2 is observed to bind Kapβ2 in an extended conformation and occupies the same PY-NLS binding site observed in other Kapβ2·PY-NLS structures.

Pubmed ID: 23535894 RIS Download

Mesh terms: Amino Acid Sequence | Binding Sites | Cell Nucleus | Crystallography, X-Ray | Humans | Hydrophobic and Hydrophilic Interactions | Molecular Sequence Data | Nuclear Localization Signals | Nucleocytoplasmic Transport Proteins | RNA, Messenger | RNA-Binding Proteins | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | beta Karyopherins

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Associated grants

  • Agency: NIBIB NIH HHS, Id: P30-EB-009998
  • Agency: NIGMS NIH HHS, Id: R01 GM069909
  • Agency: NIGMS NIH HHS, Id: R01-GM069909
  • Agency: NIGMS NIH HHS, Id: U01 GM098256
  • Agency: NIGMS NIH HHS, Id: U01 GM98256-01
  • Agency: NIGMS NIH HHS, Id: U54 GM094662

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