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Crystal structure of human Karyopherin β2 bound to the PY-NLS of Saccharomyces cerevisiae Nab2.

Import-Karyopherin or Importin proteins bind nuclear localization signals (NLSs) to mediate the import of proteins into the cell nucleus. Karyopherin β2 or Kapβ2, also known as Transportin, is a member of this transporter family responsible for the import of numerous RNA binding proteins. Kapβ2 recognizes a targeting signal termed the PY-NLS that lies within its cargos to target them through the nuclear pore complex. The recognition of PY-NLS by Kapβ2 is conserved throughout eukaryotes. Kap104, the Kapβ2 homolog in Saccharomyces cerevisiae, recognizes PY-NLSs in cargos Nab2, Hrp1, and Tfg2. We have determined the crystal structure of Kapβ2 bound to the PY-NLS of the mRNA processing protein Nab2 at 3.05-Å resolution. A seven-residue segment of the PY-NLS of Nab2 is observed to bind Kapβ2 in an extended conformation and occupies the same PY-NLS binding site observed in other Kapβ2·PY-NLS structures.

Pubmed ID: 23535894


  • Soniat M
  • Sampathkumar P
  • Collett G
  • Gizzi AS
  • Banu RN
  • Bhosle RC
  • Chamala S
  • Chowdhury S
  • Fiser A
  • Glenn AS
  • Hammonds J
  • Hillerich B
  • Khafizov K
  • Love JD
  • Matikainen B
  • Seidel RD
  • Toro R
  • Rajesh Kumar P
  • Bonanno JB
  • Chook YM
  • Almo SC


Journal of structural and functional genomics

Publication Data

June 12, 2013

Associated Grants

  • Agency: NIBIB NIH HHS, Id: P30-EB-009998
  • Agency: NIGMS NIH HHS, Id: R01 GM069909
  • Agency: NIGMS NIH HHS, Id: R01-GM069909
  • Agency: NIGMS NIH HHS, Id: U01 GM098256
  • Agency: NIGMS NIH HHS, Id: U01 GM98256-01
  • Agency: NIGMS NIH HHS, Id: U54 GM094662

Mesh Terms

  • Amino Acid Sequence
  • Binding Sites
  • Cell Nucleus
  • Crystallography, X-Ray
  • Humans
  • Hydrophobic and Hydrophilic Interactions
  • Molecular Sequence Data
  • Nuclear Localization Signals
  • Nucleocytoplasmic Transport Proteins
  • RNA, Messenger
  • RNA-Binding Proteins
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • beta Karyopherins