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Protein interaction discovery using parallel analysis of translated ORFs (PLATO).

Identifying physical interactions between proteins and other molecules is a critical aspect of biological analysis. Here we describe PLATO, an in vitro method for mapping such interactions by affinity enrichment of a library of full-length open reading frames displayed on ribosomes, followed by massively parallel analysis using DNA sequencing. We demonstrate the broad utility of the method for human proteins by identifying known and previously unidentified interacting partners of LYN kinase, patient autoantibodies, and the small-molecules gefitinib and dasatinib.

Pubmed ID: 23503679


  • Zhu J
  • Larman HB
  • Gao G
  • Somwar R
  • Zhang Z
  • Laserson U
  • Ciccia A
  • Pavlova N
  • Church G
  • Zhang W
  • Kesari S
  • Elledge SJ


Nature biotechnology

Publication Data

April 8, 2013

Associated Grants

  • Agency: NCI NIH HHS, Id: 3P30CA023100-25S8
  • Agency: NCI NIH HHS, Id: P30 CA023100
  • Agency: NCI NIH HHS, Id: U54 CA156734
  • Agency: NCI NIH HHS, Id: U54 CA156734
  • Agency: Howard Hughes Medical Institute, Id:

Mesh Terms

  • High-Throughput Nucleotide Sequencing
  • Humans
  • Open Reading Frames
  • Protein Binding
  • Protein Biosynthesis
  • Protein Interaction Mapping
  • Protein Interaction Maps
  • src-Family Kinases