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Large-scale investigation of oxygen response mutants in Saccharomyces cerevisiae.

Molecular bioSystems | Jun 8, 2013

http://www.ncbi.nlm.nih.gov/pubmed/23467670

A genome-wide screen of a yeast non-essential gene-deletion library was used to identify sick phenotypes due to oxygen deprivation. The screen provided a manageable list of 384 potentially novel as well as known oxygen responding (anoxia-survival) genes. The gene-deletion mutants were further assayed for sensitivity to ferrozine and cobalt to obtain a subset of 34 oxygen-responsive candidate genes including the known hypoxic gene activator, MGA2. With each mutant in this subset a plasmid based β-galactosidase assay was performed using the anoxic-inducible promoter from OLE1 gene, and 17 gene deletions were identified that inhibit induction under anaerobic conditions. Genetic interaction analysis for one of these mutants, the RNase-encoding POP2 gene, revealed synthetic sick interactions with a number of genes involved in oxygen sensing and response. Knockdown experiments for CNOT8, human homolog of POP2, reduced cell survival under low oxygen condition suggesting a similar function in human cells.

Pubmed ID: 23467670 RIS Download

Mesh terms: Cell Hypoxia | Cell Line | Cell Survival | Cobalt | Fatty Acid Desaturases | Ferrozine | Gene Deletion | Gene Expression Profiling | Gene Expression Regulation, Fungal | Humans | Iron Chelating Agents | Membrane Proteins | Oxygen | Promoter Regions, Genetic | Ribonucleases | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Trace Elements | Transcription Factors | Transcriptional Activation | beta-Galactosidase

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