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Cand1 promotes assembly of new SCF complexes through dynamic exchange of F box proteins.

Cell | Mar 28, 2013

http://www.ncbi.nlm.nih.gov/pubmed/23453757

The modular SCF (Skp1, cullin, and F box) ubiquitin ligases feature a large family of F box protein substrate receptors that enable recognition of diverse targets. However, how the repertoire of SCF complexes is sustained remains unclear. Real-time measurements of formation and disassembly indicate that SCF(Fbxw7) is extraordinarily stable, but, in the Nedd8-deconjugated state, the cullin-binding protein Cand1 augments its dissociation by one-million-fold. Binding and ubiquitylation assays show that Cand1 is a protein exchange factor that accelerates the rate at which Cul1-Rbx1 equilibrates with multiple F box protein-Skp1 modules. Depletion of Cand1 from cells impedes recruitment of new F box proteins to pre-existing Cul1 and profoundly alters the cellular landscape of SCF complexes. We suggest that catalyzed protein exchange may be a general feature of dynamic macromolecular machines and propose a hypothesis for how substrates, Nedd8, and Cand1 collaborate to regulate the cellular repertoire of SCF complexes.

Pubmed ID: 23453757 RIS Download

Mesh terms: Animals | Cell Line | Cullin Proteins | Escherichia coli | F-Box Proteins | Humans | Mass Spectrometry | SKP Cullin F-Box Protein Ligases | Transcription Factors

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Associated grants

  • Agency: PHS HHS, Id: 10565784
  • Agency: NCI NIH HHS, Id: CA138126
  • Agency: NIGMS NIH HHS, Id: GM065997
  • Agency: NCI NIH HHS, Id: R01 CA084069
  • Agency: NIGMS NIH HHS, Id: R01 GM065997
  • Agency: NCI NIH HHS, Id: T32 CA080416
  • Agency: Howard Hughes Medical Institute, Id:

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