• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


Fungicidal drugs induce a common oxidative-damage cellular death pathway.

Amphotericin, miconazole, and ciclopirox are antifungal agents from three different drug classes that can effectively kill planktonic yeast, yet their complete fungicidal mechanisms are not fully understood. Here, we employ a systems biology approach to identify a common oxidative-damage cellular death pathway triggered by these representative fungicides in Candida albicans and Saccharomyces cerevisiae. This mechanism utilizes a signaling cascade involving the GTPases Ras1 and Ras2 and protein kinase A, and it culminates in death through the production of toxic reactive oxygen species in a tricarboxylic-acid-cycle- and respiratory-chain-dependent manner. We also show that the metabolome of C. albicans is altered by antifungal drug treatment, exhibiting a shift from fermentation to respiration, a jump in the AMP/ATP ratio, and elevated production of sugars; this coincides with elevated mitochondrial activity. Lastly, we demonstrate that DNA damage plays a critical role in antifungal-induced cellular death and that blocking DNA-repair mechanisms potentiates fungicidal activity.

Pubmed ID: 23416050


  • Belenky P
  • Camacho D
  • Collins JJ


Cell reports

Publication Data

February 21, 2013

Associated Grants

  • Agency: NIH HHS, Id: DP1 OD003644
  • Agency: NIH HHS, Id: DP1 OD003644
  • Agency: Howard Hughes Medical Institute, Id:

Mesh Terms

  • Amphotericin B
  • Antifungal Agents
  • Candida albicans
  • Citric Acid Cycle
  • Cyclic AMP-Dependent Protein Kinases
  • DNA Damage
  • Electron Transport
  • Fungal Proteins
  • Metabolome
  • Miconazole
  • Mitochondria
  • Pyridones
  • Reactive Oxygen Species
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • ras Proteins