Literature search services are currently unavailable. During our hosting provider's UPS upgrade we experienced a hardware failure and are currently working to resolve the issue.

Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Deubiquitination of NF-κB by Ubiquitin-Specific Protease-7 promotes transcription.

NF-κB is the master regulator of the immune response and is responsible for the transcription of hundreds of genes controlling inflammation and immunity. Activation of NF-κB occurs in the cytoplasm through the kinase activity of the IκB kinase complex, which leads to translocation of NF-κB to the nucleus. Once in the nucleus, NF-κB transcriptional activity is regulated by DNA binding-dependent ubiquitin-mediated proteasomal degradation. We have identified the deubiquitinase Ubiquitin Specific Protease-7 (USP7) as a regulator of NF-κB transcriptional activity. USP7 deubiquitination of NF-κB leads to increased transcription. Loss of USP7 activity results in increased ubiquitination of NF-κB, leading to reduced promoter occupancy and reduced expression of target genes in response to Toll-like- and TNF-receptor activation. These findings reveal a unique mechanism controlling NF-κB activity and demonstrate that the deubiquitination of NF-κB by USP7 is critical for target gene transcription.

Pubmed ID: 23267096


  • Colleran A
  • Collins PE
  • O'Carroll C
  • Ahmed A
  • Mao X
  • McManus B
  • Kiely PA
  • Burstein E
  • Carmody RJ


Proceedings of the National Academy of Sciences of the United States of America

Publication Data

January 8, 2013

Associated Grants

  • Agency: NIDDK NIH HHS, Id: R01 DK073639
  • Agency: NIDDK NIH HHS, Id: R01 DK073639

Mesh Terms

  • Amino Acid Sequence
  • Animals
  • Cell Line, Tumor
  • Chromatin Immunoprecipitation
  • Gene Expression Regulation
  • HEK293 Cells
  • Humans
  • Immunoblotting
  • Immunoprecipitation
  • Mice
  • Models, Molecular
  • Molecular Sequence Data
  • NF-kappa B
  • NIH 3T3 Cells
  • Peptides
  • Real-Time Polymerase Chain Reaction
  • Transcription, Genetic
  • Transfection
  • Tumor Necrosis Factor-alpha
  • Ubiquitin Thiolesterase
  • Ubiquitination