Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

A catalytically inactive mutant of the deubiquitylase YOD-1 enhances antigen cross-presentation.

Antigen presenting cells (APCs) that express a catalytically inactive version of the deubiquitylase YOD1 (YOD1-C160S) present exogenous antigens more efficiently to CD8(+) T cells, both in vitro and in vivo. Compared with controls, immunization of YOD1-C160S mice led to greater expansion of specific CD8(+) T cells and showed improved control of infection with a recombinant -herpes virus, MHV-68, engineered to express SIINFEKL peptide, the ligand for the ovalbumin-specific TCR transgenic OT-I cells. Enhanced expansion of specific CD8(+) T cells was likewise observed on infection of YOD1-C160S mice with a recombinant influenza A virus expressing SIINFEKL. YOD1-C160S APCs retained antigen longer than did control APCs. Enhanced crosspresentation by YOD1-C160S APCs was transporter associated with antigen processing (TAP1)-independent but sensitive to inclusion of inhibitors of acidification and of the proteasome. The activity of deubiquitylating enzymes may thus help control antigenspecific CD8(+) T-cell responses during immunization.

Pubmed ID: 23243279


  • Sehrawat S
  • Koenig PA
  • Kirak O
  • Schlieker C
  • Fankhauser M
  • Ploegh HL



Publication Data

February 14, 2013

Associated Grants


Mesh Terms

  • ATP-Binding Cassette Transporters
  • Adoptive Transfer
  • Animals
  • Antigen-Presenting Cells
  • Brefeldin A
  • CD8-Positive T-Lymphocytes
  • Cross-Priming
  • Female
  • Hydrogen-Ion Concentration
  • Immunization
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Transgenic
  • Mutation, Missense
  • Ovalbumin
  • Peptide Fragments
  • Rhadinovirus
  • Ubiquitin Thiolesterase