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Global analysis of yeast mRNPs.

http://www.ncbi.nlm.nih.gov/pubmed/23222640

Proteins regulate gene expression by controlling mRNA biogenesis, localization, translation and decay. Identifying the composition, diversity and function of mRNA-protein complexes (mRNPs) is essential to understanding these processes. In a global survey of Saccharomyces cerevisiae mRNA-binding proteins, we identified 120 proteins that cross-link to mRNA, including 66 new mRNA-binding proteins. These include kinases, RNA-modification enzymes, metabolic enzymes and tRNA- and rRNA-metabolism factors. These proteins show dynamic subcellular localization during stress, including assembly into stress granules and processing bodies (P bodies). Cross-linking and immunoprecipitation (CLIP) analyses of the P-body components Pat1, Lsm1, Dhh1 and Sbp1 identified sites of interaction on specific mRNAs, revealing positional binding preferences and co-assembly preferences. When taken together, this work defines the major yeast mRNP proteins, reveals widespread changes in their subcellular location during stress and begins to define assembly rules for P-body mRNPs.

Pubmed ID: 23222640 RIS Download

Mesh terms: Binding Sites | DEAD-box RNA Helicases | Gene Expression Regulation, Fungal | RNA Cap-Binding Proteins | RNA, Fungal | RNA, Messenger | RNA-Binding Proteins | Ribonucleoproteins | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Stress, Physiological

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Associated grants

  • Agency: NCRR NIH HHS, Id: 1S10 RR028868-01
  • Agency: NIGMS NIH HHS, Id: 7R37 GM045443
  • Agency: NCI NIH HHS, Id: CA023074
  • Agency: NIEHS NIH HHS, Id: ES06694
  • Agency: NIGMS NIH HHS, Id: R37 GM045443
  • Agency: Howard Hughes Medical Institute, Id:
  • Agency: Howard Hughes Medical Institute, Id:

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