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Global analysis of yeast mRNPs.

Nature structural & molecular biology | 2013

Proteins regulate gene expression by controlling mRNA biogenesis, localization, translation and decay. Identifying the composition, diversity and function of mRNA-protein complexes (mRNPs) is essential to understanding these processes. In a global survey of Saccharomyces cerevisiae mRNA-binding proteins, we identified 120 proteins that cross-link to mRNA, including 66 new mRNA-binding proteins. These include kinases, RNA-modification enzymes, metabolic enzymes and tRNA- and rRNA-metabolism factors. These proteins show dynamic subcellular localization during stress, including assembly into stress granules and processing bodies (P bodies). Cross-linking and immunoprecipitation (CLIP) analyses of the P-body components Pat1, Lsm1, Dhh1 and Sbp1 identified sites of interaction on specific mRNAs, revealing positional binding preferences and co-assembly preferences. When taken together, this work defines the major yeast mRNP proteins, reveals widespread changes in their subcellular location during stress and begins to define assembly rules for P-body mRNPs.

Pubmed ID: 23222640 RIS Download

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Associated grants

  • Agency: NCI NIH HHS, United States
    Id: P30 CA023074
  • Agency: NCRR NIH HHS, United States
    Id: 1S10 RR028868-01
  • Agency: NCI NIH HHS, United States
    Id: CA023074
  • Agency: NIGMS NIH HHS, United States
    Id: R37 GM045443
  • Agency: NCRR NIH HHS, United States
    Id: S10 RR028868
  • Agency: NIGMS NIH HHS, United States
    Id: 7R37 GM045443
  • Agency: Howard Hughes Medical Institute, United States
  • Agency: NIEHS NIH HHS, United States
    Id: ES06694
  • Agency: NIEHS NIH HHS, United States
    Id: P30 ES006694

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UniProtKB Keywords (tool)

RRID:SCR_004313

UniProtKB entries are tagged with keywords that can be used to retrieve particular subsets of entries. There are 10 categories of keywords: Biological process Cellular component Coding sequence diversity Developmental stage Disease Domain Ligand Molecular function Post-translation modification Technical term You may browse by hierarchy, search in Keywords, or list all keywords. By default, searching the keywords will look for matches in both name and definition.

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NovoAlign (tool)

RRID:SCR_014818

Software tool designed for mapping short reads onto a reference genome generated from Illumina, Ion Torrent, and 454 NGS platforms. Its features include paired end alignment, methylation status analysis, automatic base quality calibration, and in built adapter trimming and base quality trimming.

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