• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Endocardial cells form the coronary arteries by angiogenesis through myocardial-endocardial VEGF signaling.

The origins and developmental mechanisms of coronary arteries are incompletely understood. We show here by fate mapping, clonal analysis, and immunohistochemistry that endocardial cells generate the endothelium of coronary arteries. Dye tracking, live imaging, and tissue transplantation also revealed that ventricular endocardial cells are not terminally differentiated; instead, they are angiogenic and form coronary endothelial networks. Myocardial Vegf-a or endocardial Vegfr-2 deletion inhibited coronary angiogenesis and arterial formation by ventricular endocardial cells. In contrast, lineage and knockout studies showed that endocardial cells make a small contribution to the coronary veins, the formation of which is independent of myocardial-to-endocardial Vegf signaling. Thus, contrary to the current view of a common source for the coronary vessels, our findings indicate that the coronary arteries and veins have distinct origins and are formed by different mechanisms. This information may help develop better cell therapies for coronary artery disease.

Pubmed ID: 23178125

Authors

  • Wu B
  • Zhang Z
  • Lui W
  • Chen X
  • Wang Y
  • Chamberlain AA
  • Moreno-Rodriguez RA
  • Markwald RR
  • O'Rourke BP
  • Sharp DJ
  • Zheng D
  • Lenz J
  • Baldwin HS
  • Chang CP
  • Zhou B

Journal

Cell

Publication Data

November 21, 2012

Associated Grants

  • Agency: NHLBI NIH HHS, Id: HL078881
  • Agency: NHLBI NIH HHS, Id: HL100398
  • Agency: NHLBI NIH HHS, Id: HL85345
  • Agency: NHLBI NIH HHS, Id: R01 HL078881

Mesh Terms

  • Animals
  • Cell Differentiation
  • Coronary Vessels
  • Endothelial Cells
  • Mice
  • Myocardium
  • NFATC Transcription Factors
  • Neovascularization, Physiologic
  • Signal Transduction
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factor Receptor-2