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AKAP150-anchored calcineurin regulates synaptic plasticity by limiting synaptic incorporation of Ca2+-permeable AMPA receptors.

AMPA receptors (AMPARs) are tetrameric ion channels assembled from GluA1-GluA4 subunits that mediate the majority of fast excitatory synaptic transmission in the brain. In the hippocampus, most synaptic AMPARs are composed of GluA1/2 or GluA2/3 with the GluA2 subunit preventing Ca(2+) influx. However, a small number of Ca(2+)-permeable GluA1 homomeric receptors reside in extrasynaptic locations where they can be rapidly recruited to synapses during synaptic plasticity. Phosphorylation of GluA1 S845 by the cAMP-dependent protein kinase (PKA) primes extrasynaptic receptors for synaptic insertion in response to NMDA receptor Ca(2+) signaling during long-term potentiation (LTP), while phosphatases dephosphorylate S845 and remove synaptic and extrasynaptic GluA1 during long-term depression (LTD). PKA and the Ca(2+)-activated phosphatase calcineurin (CaN) are targeted to GluA1 through binding to A-kinase anchoring protein 150 (AKAP150) in a complex with PSD-95, but we do not understand how the opposing activities of these enzymes are balanced to control plasticity. Here, we generated AKAP150ΔPIX knock-in mice to selectively disrupt CaN anchoring in vivo. We found that AKAP150ΔPIX mice lack LTD but express enhanced LTP at CA1 synapses. Accordingly, basal GluA1 S845 phosphorylation is elevated in AKAP150ΔPIX hippocampus, and LTD-induced dephosphorylation and removal of GluA1, AKAP150, and PSD-95 from synapses are impaired. In addition, basal synaptic activity of GluA2-lacking AMPARs is increased in AKAP150ΔPIX mice and pharmacologic antagonism of these receptors restores normal LTD and inhibits the enhanced LTP. Thus, AKAP150-anchored CaN opposes PKA phosphorylation of GluA1 to restrict synaptic incorporation of Ca(2+)-permeable AMPARs both basally and during LTP and LTD.

Pubmed ID: 23100425 RIS Download

Mesh terms: A Kinase Anchor Proteins | Action Potentials | Analysis of Variance | Animals | Biophysics | Calcineurin | Calcium | Cells, Cultured | Dendritic Spines | Electric Stimulation | Excitatory Amino Acid Agonists | Excitatory Amino Acid Antagonists | Excitatory Postsynaptic Potentials | Female | Guanylate Kinases | Hippocampus | Immunoprecipitation | In Vitro Techniques | Male | Membrane Proteins | Mice | Mice, Inbred C57BL | Mice, Transgenic | Mutation | N-Methylaspartate | Neuronal Plasticity | Neurons | Patch-Clamp Techniques | Phosphorylation | Quinoxalines | Receptors, AMPA | Serine | Silver Staining | Sodium Channel Blockers | Subcellular Fractions | Synapses | Tetrodotoxin

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Associated grants

  • Agency: NCRR NIH HHS, Id: UL1 RR025780
  • Agency: NIAAA NIH HHS, Id: T32 AA007464
  • Agency: NINDS NIH HHS, Id: P30 NS048154
  • Agency: NIAAA NIH HHS, Id: T32AA007464
  • Agency: NCRR NIH HHS, Id: UL1RR025780
  • Agency: NINDS NIH HHS, Id: R01 NS040701
  • Agency: NINDS NIH HHS, Id: P30NS048154

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