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Microtubule plus-end tracking protein CLASP2 regulates neuronal polarity and synaptic function.

Microtubule organization and dynamics are essential during axon and dendrite formation and maintenance in neurons. However, little is known about the regulation of microtubule dynamics during synaptic development and function in mammalian neurons. Here, we present evidence that the microtubule plus-end tracking protein CLASP2 (cytoplasmic linker associated protein 2) is a key regulator of axon and dendrite outgrowth that leads to functional alterations in synaptic activity and formation. We found that CLASP2 protein levels steadily increase throughout neuronal development in the mouse brain and are specifically enriched at the growth cones of extending neurites. The short-hairpin RNA-mediated knockdown of CLASP2 in primary mouse neurons decreased axon and dendritic length, whereas overexpression of human CLASP2 caused the formation of multiple axons, enhanced dendritic branching, and Golgi condensation, implicating CLASP2 in neuronal morphogenesis. In addition, the CLASP2-induced morphological changes led to significant functional alterations in synaptic transmission. CLASP2 overexpression produced a large increase in spontaneous miniature event frequency that was specific to excitatory neurotransmitter release. The changes in presynaptic activity produced by CLASP2 overexpression were accompanied by increases in presynaptic terminal circumference, total synapse number, and a selective increase in presynaptic proteins that are involved in neurotransmitter release. Also, we found a smaller increase in miniature event amplitude that was accompanied by an increase in postsynaptic surface expression of GluA1 receptor localization. Together, these results provide evidence for involvement of the microtubule plus-end tracking protein CLASP2 in cytoskeleton-related mechanisms underlying neuronal polarity and interplay between microtubule stabilization and synapse formation and activity.

Pubmed ID: 23035100


  • Beffert U
  • Dillon GM
  • Sullivan JM
  • Stuart CE
  • Gilbert JP
  • Kambouris JA
  • Ho A


The Journal of neuroscience : the official journal of the Society for Neuroscience

Publication Data

October 3, 2012

Associated Grants

  • Agency: NIA NIH HHS, Id: K01 AG027311
  • Agency: NIA NIH HHS, Id: K01 AG027311

Mesh Terms

  • Animals
  • Axons
  • Cell Polarity
  • Cells, Cultured
  • Cytoskeleton
  • Dendrites
  • Female
  • Golgi Apparatus
  • Growth Cones
  • Humans
  • Male
  • Mice
  • Microtubule-Associated Proteins
  • Microtubules
  • Morphogenesis
  • Nerve Tissue Proteins
  • Neurogenesis
  • Neurons
  • Neurotransmitter Agents
  • Phosphatidylinositol 3-Kinases
  • Presynaptic Terminals
  • RNA Interference
  • RNA, Small Interfering
  • Recombinant Fusion Proteins
  • Synaptic Transmission