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MRN1 implicates chromatin remodeling complexes and architectural factors in mRNA maturation.

A functional relationship between chromatin structure and mRNA processing events has been suggested, however, so far only a few involved factors have been characterized. Here we show that rsc nhp6ΔΔ mutants, deficient for the function of the chromatin remodeling factor RSC and the chromatin architectural proteins Nhp6A/Nhp6B, accumulate intron-containing pre-mRNA at the restrictive temperature. In addition, we demonstrate that rsc8-ts16 nhp6ΔΔ cells contain low levels of U6 snRNA and U4/U6 di-snRNA that is further exacerbated after two hours growth at the restrictive temperature. This change in U6 snRNA and U4/U6 di-snRNA levels in rsc8-ts16 nhp6ΔΔ cells is indicative of splicing deficient conditions. We identify MRN1 (multi-copy suppressor of rsc nhp6ΔΔ) as a growth suppressor of rsc nhp6ΔΔ synthetic sickness. Mrn1 is an RNA binding protein that localizes both to the nucleus and cytoplasm. Genetic interactions are observed between 2 µm-MRN1 and the splicing deficient mutants snt309Δ, prp3, prp4, and prp22, and additional genetic analyses link MRN1, SNT309, NHP6A/B, SWI/SNF, and RSC supporting the notion of a role of chromatin structure in mRNA processing.

Pubmed ID: 23028530


  • Düring L
  • Thorsen M
  • Petersen DS
  • Køster B
  • Jensen TH
  • Holmberg S


PloS one

Publication Data

October 2, 2012

Associated Grants


Mesh Terms

  • Chromatin Assembly and Disassembly
  • DNA-Binding Proteins
  • HMGN Proteins
  • Immunoblotting
  • Microscopy, Fluorescence
  • RNA, Messenger
  • RNA, Small Nuclear
  • Reverse Transcriptase Polymerase Chain Reaction
  • Saccharomyces cerevisiae Proteins
  • Spliceosomes
  • Transcription Factors