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IκB kinase ε phosphorylates TRAF2 to promote mammary epithelial cell transformation.

NF-κB transcription factors are central regulators of inflammation and when dysregulated contribute to malignant transformation. IκB kinase ε (IKKε; IKKi, encoded by IKBKE) is a breast oncogene that is amplified in 30% of breast cancers and drives transformation in an NF-κB-dependent manner. Here we demonstrate that IKKε interacts with and phosphorylates tumor necrosis factor receptor-associated factor 2 (TRAF2) at Ser11 in vitro and in vivo. This activity promotes Lys63-linked TRAF2 ubiquitination and NF-κB activation and is essential for IKKε transformation. Breast cancer cells that depend on IKKε expression for survival are also dependent on TRAF2. This work defines TRAF2 phosphorylation to be one key effector of IKKε-induced mammary epithelial cell transformation.

Pubmed ID: 23007157 RIS Download

Mesh terms: Amino Acid Sequence | Animals | Breast | Breast Neoplasms | Cell Line, Tumor | Cell Transformation, Neoplastic | Cysteine Endopeptidases | Down-Regulation | Epithelial Cells | Female | HEK293 Cells | Humans | I-kappa B Kinase | Mice | NF-kappa B | NIH 3T3 Cells | Nuclear Pore Complex Proteins | Phosphorylation | RNA-Binding Proteins | TNF Receptor-Associated Factor 2 | Ubiquitination

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Associated grants

  • Agency: NCI NIH HHS, Id: F32 CA128265
  • Agency: NCI NIH HHS, Id: P01 CA154303
  • Agency: NCI NIH HHS, Id: R01 CA130988
  • Agency: NCI NIH HHS, Id: R01 CA138475

BioGRID (Data, Interactions)

Addgene (Reagent, Plasmid)

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