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Hog1 controls global reallocation of RNA Pol II upon osmotic shock in Saccharomyces cerevisiae.

G3 (Bethesda, Md.) | Sep 13, 2012

When challenged with osmotic shock, Saccharomyces cerevisiae induces hundreds of genes, despite a concurrent reduction in overall transcriptional capacity. The stress-responsive MAP kinase Hog1 activates expression of specific genes through interactions with chromatin remodeling enzymes, transcription factors, and RNA polymerase II. However, it is not clear whether Hog1 is involved more globally in modulating the cell's transcriptional program during stress, in addition to activating specific genes. Here we show that large-scale redistribution of RNA Pol II from housekeeping to stress genes requires Hog1. We demonstrate that decreased RNA Pol II occupancy is the default outcome for highly expressed genes upon stress and that Hog1 is partially required for this effect. We find that Hog1 and RNA Pol II colocalize to open reading frames that bypass global transcriptional repression. These activation targets are specified by promoter binding of two osmotic stress-responsive transcription factors. The combination of reduced global transcription with a gene-specific override mechanism allows cells to rapidly switch their transcriptional program in response to stress.

Pubmed ID: 22973550 RIS Download

Mesh terms: Basic-Leucine Zipper Transcription Factors | Binding Sites | Mitogen-Activated Protein Kinases | Open Reading Frames | Osmotic Pressure | Promoter Regions, Genetic | Protein Binding | Protein Transport | RNA Polymerase II | Regulatory Sequences, Nucleic Acid | Repressor Proteins | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Stress, Physiological | Transcription Factors

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Associated grants

  • Agency: Howard Hughes Medical Institute, Id:

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SGD

A curated database that provides comprehensive integrated biological information for Saccharomyces cerevisiae along with search and analysis tools to explore these data. SGD allows researchers to discover functional relationships between sequence and gene products in fungi and higher organisms. The SGD also maintains the S. cerevisiae Gene Name Registry, a complete list of all gene names used in S. cerevisiae which includes a set of general guidelines to gene naming. Protein Page provides basic protein information calculated from the predicted sequence and contains links to a variety of secondary structure and tertiary structure resources. Yeast Biochemical Pathways allows users to view and search for biochemical reactions and pathways that occur in S. cerevisiae as well as map expression data onto the biochemical pathways. Literature citations are provided where available.

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