• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

MicroRNA-10 regulates the angiogenic behavior of zebrafish and human endothelial cells by promoting vascular endothelial growth factor signaling.

RATIONALE: Formation and remodeling of the vasculature during development and disease involve a highly conserved and precisely regulated network of attractants and repellants. Various signaling pathways control the behavior of endothelial cells, but their posttranscriptional dose titration by microRNAs is poorly understood. OBJECTIVE: To identify microRNAs that regulate angiogenesis. METHODS AND RESULTS: We show that the highly conserved microRNA family encoding miR-10 regulates the behavior of endothelial cells during angiogenesis by positively titrating proangiogenic signaling. Knockdown of miR-10 led to premature truncation of intersegmental vessel growth in the trunk of zebrafish larvae, whereas overexpression of miR-10 promoted angiogenic behavior in zebrafish and cultured human umbilical venous endothelial cells. We found that miR-10 functions, in part, by directly regulating the level of fms-related tyrosine kinase 1 (FLT1), a cell-surface protein that sequesters vascular endothelial growth factor, and its soluble splice variant sFLT1. The increase in FLT1/sFLT1 protein levels upon miR-10 knockdown in zebrafish and in human umbilical venous endothelial cells inhibited the angiogenic behavior of endothelial cells largely by antagonizing vascular endothelial growth factor receptor 2 signaling. CONCLUSIONS: Our study provides insights into how FLT1 and vascular endothelial growth factor receptor 2 signaling is titrated in a microRNA-mediated manner and establishes miR-10 as a potential new target for the selective modulation of angiogenesis.

Pubmed ID: 22955733

Authors

  • Hassel D
  • Cheng P
  • White MP
  • Ivey KN
  • Kroll J
  • Augustin HG
  • Katus HA
  • Stainier DY
  • Srivastava D

Journal

Circulation research

Publication Data

November 9, 2012

Associated Grants

  • Agency: NCRR NIH HHS, Id: C06 RR018928
  • Agency: NHLBI NIH HHS, Id: HL54737
  • Agency: NHLBI NIH HHS, Id: P01 HL089707
  • Agency: NHLBI NIH HHS, Id: R01 HL057181
  • Agency: NIGMS NIH HHS, Id: T32 GM007618
  • Agency: PHS HHS, Id: T32 GMO7618
  • Agency: NHLBI NIH HHS, Id: U01 HL100406
  • Agency: NHLBI NIH HHS, Id: U01 HL100406

Mesh Terms

  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • Cell Proliferation
  • Cells, Cultured
  • Endothelial Cells
  • Female
  • Gene Knockdown Techniques
  • Green Fluorescent Proteins
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Immunoblotting
  • Larva
  • Male
  • Mice
  • MicroRNAs
  • Microscopy, Confocal
  • Neovascularization, Physiologic
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Nucleic Acid
  • Signal Transduction
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factor Receptor-1
  • Vascular Endothelial Growth Factor Receptor-2
  • Zebrafish