Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

A census of human soluble protein complexes.

Cell | Aug 31, 2012

http://www.ncbi.nlm.nih.gov/pubmed/22939629

Cellular processes often depend on stable physical associations between proteins. Despite recent progress, knowledge of the composition of human protein complexes remains limited. To close this gap, we applied an integrative global proteomic profiling approach, based on chromatographic separation of cultured human cell extracts into more than one thousand biochemical fractions that were subsequently analyzed by quantitative tandem mass spectrometry, to systematically identify a network of 13,993 high-confidence physical interactions among 3,006 stably associated soluble human proteins. Most of the 622 putative protein complexes we report are linked to core biological processes and encompass both candidate disease genes and unannotated proteins to inform on mechanism. Strikingly, whereas larger multiprotein assemblies tend to be more extensively annotated and evolutionarily conserved, human protein complexes with five or fewer subunits are far more likely to be functionally unannotated or restricted to vertebrates, suggesting more recent functional innovations.

Pubmed ID: 22939629 RIS Download

Mesh terms: Humans | Multiprotein Complexes | Protein Interaction Maps | Proteins | Proteomics | Tandem Mass Spectrometry

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: Biotechnology and Biological Sciences Research Council, Id: BB/F00964X/1
  • Agency: Biotechnology and Biological Sciences Research Council, Id: BB/K004131/1
  • Agency: NIGMS NIH HHS, Id: DP1 GM106408
  • Agency: Canadian Institutes of Health Research, Id: MOP 82940
  • Agency: NIGMS NIH HHS, Id: R01 GM067779
  • Agency: NIGMS NIH HHS, Id: R01 GM076536
  • Agency: NIGMS NIH HHS, Id: R01 GM088624

BioGRID (Data, Interactions)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.