Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Yeast Trm7 interacts with distinct proteins for critical modifications of the tRNAPhe anticodon loop.

RNA (New York, N.Y.) | Oct 18, 2012

http://www.ncbi.nlm.nih.gov/pubmed/22912484

Post-transcriptional modification of the tRNA anticodon loop is critical for translation. Yeast Trm7 is required for 2'-O-methylation of C(32) and N(34) of tRNA(Phe), tRNA(Trp), and tRNA(Leu(UAA)) to form Cm(32) and Nm(34), and trm7-Δ mutants have severe growth and translation defects, but the reasons for these defects are not known. We show here that overproduction of tRNA(Phe) suppresses the growth defect of trm7-Δ mutants, suggesting that the crucial biological role of Trm7 is the modification of tRNA(Phe). We also provide in vivo and in vitro evidence that Trm7 interacts with ORF YMR259c (now named Trm732) for 2'-O-methylation of C(32), and with Rtt10 (named Trm734) for 2'-O-methylation of N(34) of substrate tRNAs and provide evidence for a complex circuitry of anticodon loop modification of tRNA(Phe), in which formation of Cm(32) and Gm(34) drives modification of m(1)G(37) (1-methylguanosine) to yW (wyebutosine). Further genetic analysis shows that the slow growth of trm7-Δ mutants is due to the lack of both Cm(32) and Nm(34), and the accompanying loss of yW, because trm732-Δ trm734-Δ mutants phenocopy trm7-Δ mutants, whereas each single mutant is healthy; nonetheless, TRM732 and TRM734 each have distinct roles, since mutations in these genes have different genetic interactions with trm1-Δ mutants, which lack m(2,2)G(26) in their tRNAs. We speculate that 2'-O-methylation of the anticodon loop may be important throughout eukaryotes because of the widespread conservation of Trm7, Trm732, and Trm734 proteins, and the corresponding modifications, and because the putative human TRM7 ortholog FTSJ1 is implicated in nonsyndromic X-linked mental retardation.

Pubmed ID: 22912484 RIS Download

Mesh terms: Anticodon | Base Sequence | Carrier Proteins | Nucleic Acid Conformation | Organisms, Genetically Modified | Protein Binding | RNA Processing, Post-Transcriptional | RNA, Transfer, Phe | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Vesicular Transport Proteins | Yeasts | tRNA Methyltransferases

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIGMS NIH HHS, Id: 5T32 GM068411
  • Agency: NIGMS NIH HHS, Id: GM27930
  • Agency: NIGMS NIH HHS, Id: GM52347
  • Agency: NIGMS NIH HHS, Id: R01 GM027930
  • Agency: NCI NIH HHS, Id: T32 CA09363
  • Agency: NIGMS NIH HHS, Id: T32 GM068411

BioGRID (Data, Interactions)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.