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Proteomic screen reveals Fbw7 as a modulator of the NF-κB pathway.

Nature communications | Aug 6, 2012

Fbw7 is a ubiquitin-ligase that targets several oncoproteins for proteolysis, but the full range of Fbw7 substrates is not known. Here we show that by performing quantitative proteomics combined with degron motif searches, we effectively screened for a more complete set of Fbw7 targets. We identify 89 putative Fbw7 substrates, including several disease-associated proteins. The transcription factor NF-κB2 (p100/p52) is one of the candidate Fbw7 substrates. We show that Fbw7 interacts with p100 via a conserved degron and that it promotes degradation of p100 in a GSK3β phosphorylation-dependent manner. Fbw7 inactivation increases p100 levels, which in the presence of NF-κB pathway stimuli, leads to increased p52 levels and activity. Accordingly, the apoptotic threshold can be increased by loss of Fbw7 in a p100-dependent manner. In conclusion, Fbw7-mediated destruction of p100 is a regulatory component restricting the response to NF-κB2 pathway stimulation.

Pubmed ID: 22864569 RIS Download

Mesh terms: Cell Cycle Proteins | Cell Line | Cell Line, Tumor | Computational Biology | F-Box Proteins | Glycogen Synthase Kinase 3 | Humans | Immunoblotting | Immunoprecipitation | NF-kappa B p52 Subunit | Phosphorylation | Proteomics | Signal Transduction | Tandem Mass Spectrometry | Ubiquitin-Protein Ligases

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Associated grants

  • Agency: Cancer Research UK, Id: 12918
  • Agency: Cancer Research UK, Id: A12918
  • Agency: NCI NIH HHS, Id: R01 CA116616
  • Agency: Cancer Research UK, Id:

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UniProt

Resource for protein sequence and functional information that welcomes updates, corrections, and the submission of new protein sequence data. It is comprised of four components, each optimized for different uses. UniProt Knowledgebase (UniProtKB) is the central access point for curated protein information, including function, classification, and cross-reference, and contains two sections: UniProtKB/Swiss-Prot, which is manually annotated and reviewed; and UniProtKB/TrEMBL, which is automatically annotated and not reviewed. UniProt Reference Clusters (UniRef) are databases providing clustered sets of sequences from the UniProtKB and selected UniProt Archive records to obtain complete coverage of sequence space at several resolutions while hiding redundant sequences. UniProt Archive (UniParc) is a comprehensive repository used to keep track of sequences and their identifiers. UniProt Metagenomic and Environmental Sequences (UniMES) database is a repository specifically developed for metagenomic and environmental data.

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