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The innate immune sensor NLRC3 attenuates Toll-like receptor signaling via modification of the signaling adaptor TRAF6 and transcription factor NF-κB.

Nature immunology | Sep 23, 2012

Several members of the NLR family of sensors activate innate immunity. In contrast, we found here that NLRC3 inhibited Toll-like receptor (TLR)-dependent activation of the transcription factor NF-κB by interacting with the TLR signaling adaptor TRAF6 to attenuate Lys63 (K63)-linked ubiquitination of TRAF6 and activation of NF-κB. We used bioinformatics to predict interactions between NLR and TRAF proteins, including interactions of TRAF with NLRC3. In vivo, macrophage expression of Nlrc3 mRNA was diminished by the administration of lipopolysaccharide (LPS) but was restored when cellular activation subsided. To assess biologic relevance, we generated Nlrc3(-/-) mice. LPS-treated Nlrc3(-/-) macrophages had more K63-ubiquitinated TRAF6, nuclear NF-κB and proinflammatory cytokines. Finally, LPS-treated Nlrc3(-/-) mice had more signs of inflammation. Thus, signaling via NLRC3 and TLR constitutes a negative feedback loop. Furthermore, prevalent NLR-TRAF interactions suggest the formation of a 'TRAFasome' complex.

Pubmed ID: 22863753 RIS Download

Mesh terms: Amino Acid Sequence | Animals | Feedback, Physiological | HEK293 Cells | Humans | Macrophages | Mice | Mice, Inbred C57BL | Mice, Knockout | Molecular Sequence Data | NF-kappa B | Real-Time Polymerase Chain Reaction | Receptors, G-Protein-Coupled | Reverse Transcriptase Polymerase Chain Reaction | Signal Transduction | TNF Receptor-Associated Factor 6 | Toll-Like Receptors

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Associated grants

  • Agency: NIAID NIH HHS, Id: R37-AI029564
  • Agency: NIAID NIH HHS, Id: U19-AI1077437
  • Agency: NIAMS NIH HHS, Id: T32-AR007416
  • Agency: NIDDK NIH HHS, Id: K01 DK092355
  • Agency: NIAMS NIH HHS, Id: T32 AR007416
  • Agency: NIAID NIH HHS, Id: U19 AI077437
  • Agency: NIAID NIH HHS, Id: U54-AI057157
  • Agency: NIAID NIH HHS, Id: R37 AI029564
  • Agency: NIAID NIH HHS, Id: U54 AI057157

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