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Systematic functional prioritization of protein posttranslational modifications.

Cell | 2012

Protein function is often regulated by posttranslational modifications (PTMs), and recent advances in mass spectrometry have resulted in an exponential increase in PTM identification. However, the functional significance of the vast majority of these modifications remains unknown. To address this problem, we compiled nearly 200,000 phosphorylation, acetylation, and ubiquitination sites from 11 eukaryotic species, including 2,500 newly identified ubiquitylation sites for Saccharomyces cerevisiae. We developed methods to prioritize the functional relevance of these PTMs by predicting those that likely participate in cross-regulatory events, regulate domain activity, or mediate protein-protein interactions. PTM conservation within domain families identifies regulatory "hot spots" that overlap with functionally important regions, a concept that we experimentally validated on the HSP70 domain family. Finally, our analysis of the evolution of PTM regulation highlights potential routes for neutral drift in regulatory interactions and suggests that only a fraction of modification sites are likely to have a significant biological role.

Pubmed ID: 22817900 RIS Download

Research resources used in this publication

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Associated grants

  • Agency: NIGMS NIH HHS, United States
    Id: GM56433
  • Agency: NIGMS NIH HHS, United States
    Id: GM082250
  • Agency: NIGMS NIH HHS, United States
    Id: GM062583
  • Agency: NIGMS NIH HHS, United States
    Id: GM081879
  • Agency: NIGMS NIH HHS, United States
    Id: P50 GM081879
  • Agency: NIGMS NIH HHS, United States
    Id: R01 GM055040
  • Agency: NIAID NIH HHS, United States
    Id: AI090935
  • Agency: NIGMS NIH HHS, United States
    Id: R01 GM056433
  • Agency: NCRR NIH HHS, United States
    Id: P41 RR001614
  • Agency: Howard Hughes Medical Institute, United States
  • Agency: NIAID NIH HHS, United States
    Id: P01 AI090935
  • Agency: NIGMS NIH HHS, United States
    Id: GM55040
  • Agency: NIGMS NIH HHS, United States
    Id: GM084279
  • Agency: NIGMS NIH HHS, United States
    Id: GM084448
  • Agency: NCRR NIH HHS, United States
    Id: RR01614
  • Agency: NIGMS NIH HHS, United States
    Id: R01 GM062583
  • Agency: NEI NIH HHS, United States
    Id: PN2 EY016546
  • Agency: NIGMS NIH HHS, United States
    Id: P50 GM082250
  • Agency: NIAID NIH HHS, United States
    Id: P30 AI027763
  • Agency: NIGMS NIH HHS, United States
    Id: R01 GM084448
  • Agency: NIH HHS, United States
    Id: DP5 OD009180
  • Agency: NIGMS NIH HHS, United States
    Id: R01 GM084279
  • Agency: NIGMS NIH HHS, United States
    Id: R37 GM056433

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