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Sphingosine-1-phosphate lyase expression in embryonic and adult murine tissues.

Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid involved in immunity, inflammation, angiogenesis, and cancer. S1P lyase (SPL) is the essential enzyme responsible for S1P degradation. SPL augments apoptosis and is down-regulated in cancer. SPL generates a S1P chemical gradient that promotes lymphocyte trafficking and as such is being targeted to treat autoimmune diseases. Despite growing interest in SPL as a disease marker, antioncogene, and pharmacological target, no comprehensive characterization of SPL expression in mammalian tissues has been reported. We investigated SPL expression in developing and adult mouse tissues by generating and characterizing a β-galactosidase-SPL reporter mouse combined with immunohistochemistry, immunoblotting, and enzyme assays. SPL was expressed in thymic and splenic stromal cells, splenocytes, Peyer's Patches, colonic lymphoid aggregates, circulating T and B lymphocytes, granulocytes, and monocytes, with lowest expression in thymocytes. SPL was highly expressed within the CNS, including arachnoid lining cells, spinal cord, choroid plexus, trigeminal nerve ganglion, and specific neurons of the olfactory bulb, cerebral cortex, midbrain, hindbrain, and cerebellum. Expression was detected in brown adipose tissue, female gonads, adrenal cortex, bladder epithelium, Harderian and preputial glands, and hair follicles. This unique expression pattern suggests SPL has many undiscovered physiological functions apart from its role in immunity.

Pubmed ID: 22781001


  • Borowsky AD
  • Bandhuvula P
  • Kumar A
  • Yoshinaga Y
  • Nefedov M
  • Fong LG
  • Zhang M
  • Baridon B
  • Dillard L
  • de Jong P
  • Young SG
  • West DB
  • Saba JD


Journal of lipid research

Publication Data

September 8, 2012

Associated Grants

  • Agency: NCI NIH HHS, Id: CA129438
  • Agency: NCI NIH HHS, Id: CA77528
  • Agency: NIGMS NIH HHS, Id: GM66152
  • Agency: NIGMS NIH HHS, Id: GM66954
  • Agency: NHLBI NIH HHS, Id: HL76839
  • Agency: NHLBI NIH HHS, Id: HL86683
  • Agency: NCRR NIH HHS, Id: K26 RR024037
  • Agency: NHLBI NIH HHS, Id: P01 HL090553
  • Agency: NHLBI NIH HHS, Id: P01 HL090553
  • Agency: NCI NIH HHS, Id: P30 CA093373
  • Agency: NIGMS NIH HHS, Id: R01 GM066954
  • Agency: NHLBI NIH HHS, Id: R01 HL087228
  • Agency: NCI NIH HHS, Id: U01 CA14582
  • Agency: NIH HHS, Id: U42 OD011175
  • Agency: NCRR NIH HHS, Id: U42RR033193
  • Agency: NHGRI NIH HHS, Id: U54 HG006364
  • Agency: NHGRI NIH HHS, Id: U54HG006364

Mesh Terms

  • Aldehyde-Lyases
  • Animals
  • Embryo, Mammalian
  • Female
  • Gene Expression Regulation, Developmental
  • Genes, Reporter
  • Male
  • Mice
  • Mutation
  • Organ Specificity
  • beta-Galactosidase