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Sphingosine-1-phosphate lyase expression in embryonic and adult murine tissues.

Journal of lipid research | 2012

Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid involved in immunity, inflammation, angiogenesis, and cancer. S1P lyase (SPL) is the essential enzyme responsible for S1P degradation. SPL augments apoptosis and is down-regulated in cancer. SPL generates a S1P chemical gradient that promotes lymphocyte trafficking and as such is being targeted to treat autoimmune diseases. Despite growing interest in SPL as a disease marker, antioncogene, and pharmacological target, no comprehensive characterization of SPL expression in mammalian tissues has been reported. We investigated SPL expression in developing and adult mouse tissues by generating and characterizing a β-galactosidase-SPL reporter mouse combined with immunohistochemistry, immunoblotting, and enzyme assays. SPL was expressed in thymic and splenic stromal cells, splenocytes, Peyer's Patches, colonic lymphoid aggregates, circulating T and B lymphocytes, granulocytes, and monocytes, with lowest expression in thymocytes. SPL was highly expressed within the CNS, including arachnoid lining cells, spinal cord, choroid plexus, trigeminal nerve ganglion, and specific neurons of the olfactory bulb, cerebral cortex, midbrain, hindbrain, and cerebellum. Expression was detected in brown adipose tissue, female gonads, adrenal cortex, bladder epithelium, Harderian and preputial glands, and hair follicles. This unique expression pattern suggests SPL has many undiscovered physiological functions apart from its role in immunity.

Pubmed ID: 22781001 RIS Download

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Associated grants

  • Agency: NIGMS NIH HHS, United States
    Id: GM66954
  • Agency: NCRR NIH HHS, United States
    Id: U42 RR033193
  • Agency: NHGRI NIH HHS, United States
    Id: U54 HG006364
  • Agency: NHLBI NIH HHS, United States
    Id: HL76839
  • Agency: NIH HHS, United States
    Id: U42 OD011175
  • Agency: NHLBI NIH HHS, United States
    Id: R01 HL076839
  • Agency: NCI NIH HHS, United States
    Id: R01 CA077528
  • Agency: NIGMS NIH HHS, United States
    Id: GM66152
  • Agency: NCI NIH HHS, United States
    Id: R01 CA129438
  • Agency: NIGMS NIH HHS, United States
    Id: R01 GM066954
  • Agency: NCI NIH HHS, United States
    Id: P30 CA093373
  • Agency: NHLBI NIH HHS, United States
    Id: R01 HL087228
  • Agency: NHGRI NIH HHS, United States
    Id: U54HG006364
  • Agency: NCI NIH HHS, United States
    Id: CA77528
  • Agency: NHLBI NIH HHS, United States
    Id: R01 HL086683
  • Agency: NHLBI NIH HHS, United States
    Id: P01 HL090553
  • Agency: NCI NIH HHS, United States
    Id: CA129438
  • Agency: NIGMS NIH HHS, United States
    Id: R01 GM066152
  • Agency: NCRR NIH HHS, United States
    Id: U42RR033193
  • Agency: NCRR NIH HHS, United States
    Id: K26 RR024037
  • Agency: NCI NIH HHS, United States
    Id: U01 CA14582
  • Agency: NHLBI NIH HHS, United States
    Id: HL86683

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International Mouse Phenotyping Consortium (IMPC) (tool)

RRID:SCR_006158

Center that produces knockout mice and carries out high-throughput phenotyping of each line in order to determine function of every gene in mouse genome. These mice will be preserved in repositories and made available to scientific community representing valuable resource for basic scientific research as well as generating new models for human diseases.

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