Defect in regulatory B-cell function and development of systemic autoimmunity in T-cell Ig mucin 1 (Tim-1) mucin domain-mutant mice.
Tim-1, a type I transmembrane glycoprotein, consists of an IgV domain and a mucin domain. The IgV domain is essential for binding Tim-1 to its ligands, but little is known about the role of the mucin domain, even though genetic association of TIM-1 with atopy/asthma has been linked to the length of mucin domain. We generated a Tim-1-mutant mouse (Tim-1(Δmucin)) in which the mucin domain was deleted genetically. The mutant mice showed a profound defect in IL-10 production from regulatory B cells (Bregs). Associated with the loss of IL-10 production in B cells, older Tim-1(Δmucin) mice developed spontaneous autoimmunity associated with hyperactive T cells, with increased production of IFN-γ and elevated serum levels of Ig and autoantibodies. However, Tim-1(Δmucin) mice did not develop frank systemic autoimmune disease unless they were crossed onto the Fas-mutant lpr mice on a C57BL/6 background. Tim-1(Δmucin)lpr mice developed accelerated and fulminant systemic autoimmunity with accumulation of abnormal double-negative T cells and autoantibodies to a number of lupus-associated autoantigens. Thus, Tim-1 plays a critical role in maintaining suppressive Breg function, and our data also demonstrate an unexpected role of the Tim-1 mucin domain in regulating Breg function and maintaining self-tolerance.
Pubmed ID: 22773818 RIS Download
Animals | Autoantibodies | Autoimmunity | B-Lymphocytes, Regulatory | Blotting, Western | Crosses, Genetic | DNA Primers | Enzyme-Linked Immunosorbent Assay | Flow Cytometry | Hepatitis A Virus Cellular Receptor 1 | Interferon-gamma | Interleukin-10 | Membrane Proteins | Mice | Mice, Inbred C57BL | Mice, Mutant Strains | Mucins | Mutagenesis | Protein Structure, Tertiary | Reverse Transcriptase Polymerase Chain Reaction | T-Lymphocytes