We have updated our privacy policy. If you have any question, contact us at privacy@scicrunch.org. Dismiss and don't show again

Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Architecture of the RNA polymerase II preinitiation complex and mechanism of ATP-dependent promoter opening.

Yeast RNA polymerase II (Pol II) general transcription factor TFIIE and the TFIIH subunit Ssl2 (yeast ortholog of mammalian XPB) function in the transition of the preinitiation complex (PIC) to the open complex. We show that the three TFIIE winged-helix (WH) domains form a heterodimer, with the Tfa1 (TFIIEα) WH binding the Pol II clamp and the Tfa2 (TFIIEβ) tandem WH domain encircling promoter DNA that becomes single-stranded in the open complex. Ssl2 lies adjacent to TFIIE, enclosing downstream promoter DNA. Unlike previous proposals, comparison of the PIC and open-complex models strongly suggests that Ssl2 promotes DNA opening by functioning as a double-stranded-DNA translocase, feeding 15 base pairs into the Pol II cleft. Right-handed threading of DNA through the Ssl2 binding groove, combined with the fixed position of upstream promoter DNA, leads to DNA unwinding and the open state.

Pubmed ID: 22751016 RIS Download

Mesh terms: Adenosine Triphosphate | Binding Sites | DNA Helicases | DNA, Fungal | Dimerization | Models, Molecular | Multiprotein Complexes | Mutagenesis, Site-Directed | Nucleic Acid Conformation | Nucleic Acid Denaturation | Promoter Regions, Genetic | Protein Interaction Domains and Motifs | RNA Polymerase II | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Transcription Factor TFIIH | Transcription Factors, TFII

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

Associated grants

  • Agency: NIGMS NIH HHS, Id: R01 GM053451
  • Agency: NIGMS NIH HHS, Id: R01GM053451

BioGRID (Data, Interactions)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.