• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Uropod elongation is a common final step in leukocyte extravasation through inflamed vessels.

The efficient trafficking of immune cells into peripheral nonlymphoid tissues is key to enact their protective functions. Despite considerable advances in our understanding of cell migration in secondary lymphoid organs, real-time leukocyte recruitment into inflamed tissues is not well characterized. The conventional multistep paradigm of leukocyte extravasation depends on CD18 integrin-mediated events such as rapid arrest and crawling on the surface of the endothelium and transmigration through the endothelial layer. Using enhanced three-dimensional detection of fluorescent CD18 fusion proteins in a newly developed knockin mouse, we report that extravasating leukocytes (neutrophils, monocytes, and T cells) show delayed uropod detachment and become extremely elongated before complete transmigration across the endothelium. Additionally, these cells deposit CD18(+) microparticles at the subendothelial layer before retracting the stretched uropod. Experiments with knockout mice and blocking antibodies reveal that the uropod elongation and microparticle formation are the result of LFA-1-mediated adhesion and VLA-3-mediated cell migration through the vascular basement membrane. These findings suggest that uropod elongation is a final step in the leukocyte extravasation cascade, which may be important for precise regulation of leukocyte recruitment into inflamed tissues.

Pubmed ID: 22711877

Authors

  • Hyun YM
  • Sumagin R
  • Sarangi PP
  • Lomakina E
  • Overstreet MG
  • Baker CM
  • Fowell DJ
  • Waugh RE
  • Sarelius IH
  • Kim M

Journal

The Journal of experimental medicine

Publication Data

July 2, 2012

Associated Grants

  • Agency: NHLBI NIH HHS, Id: HL018208
  • Agency: NHLBI NIH HHS, Id: HL087088
  • Agency: NHLBI NIH HHS, Id: P01 HL018208
  • Agency: NIAID NIH HHS, Id: R01 AI072690
  • Agency: NHLBI NIH HHS, Id: R01 HL087088

Mesh Terms

  • Animals
  • Antigens, CD18
  • Cell Adhesion
  • Cell Surface Extensions
  • Cells, Cultured
  • Endothelium, Vascular
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Integrin alpha3beta1
  • Leukocytes
  • Luminescent Proteins
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Transgenic
  • Microscopy, Electron
  • Microscopy, Fluorescence
  • Neutrophils
  • T-Lymphocytes
  • Transendothelial and Transepithelial Migration
  • Vasculitis