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Structure of Mre11-Nbs1 complex yields insights into ataxia-telangiectasia-like disease mutations and DNA damage signaling.

http://www.ncbi.nlm.nih.gov/pubmed/22705791

The Mre11-Rad50-Nbs1 (MRN) complex tethers, processes and signals DNA double-strand breaks, promoting genomic stability. To understand the functional architecture of MRN, we determined the crystal structures of the Schizosaccharomyces pombe Mre11 dimeric catalytic domain alone and in complex with a fragment of Nbs1. Two Nbs1 subunits stretch around the outside of the nuclease domains of Mre11, with one subunit additionally bridging and locking the Mre11 dimer via a highly conserved asymmetrical binding motif. Our results show that Mre11 forms a flexible dimer and suggest that Nbs1 not only is a checkpoint adaptor but also functionally influences Mre11-Rad50. Clinical mutations in Mre11 are located along the Nbs1-interaction sites and weaken the Mre11-Nbs1 interaction. However, they differentially affect DNA repair and telomere maintenance in Saccharomyces cerevisiae, potentially providing insight into their different human disease pathologies.

Pubmed ID: 22705791 RIS Download

Mesh terms: Binding Sites | Chromosomal Proteins, Non-Histone | DNA Damage | Dimerization | Humans | Models, Molecular | Mutation | Protein Conformation | Schizosaccharomyces pombe Proteins | Signal Transduction

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Associated grants

  • Agency: Wellcome Trust, Id: 092096
  • Agency: Cancer Research UK, Id: 11224
  • Agency: European Research Council, Id: 268536
  • Agency: Cancer Research UK, Id: A11224
  • Agency: Cancer Research UK, Id: C6/A11226
  • Agency: NIAID NIH HHS, Id: U19 AI083025
  • Agency: NIAID NIH HHS, Id: U19AI83025
  • Agency: Wellcome Trust, Id:

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