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A ubiquitin-binding protein, FAAP20, links RNF8-mediated ubiquitination to the Fanconi anemia DNA repair network.

The Fanconi anemia (FA) protein network is necessary for repair of DNA interstrand crosslinks (ICLs), but its control mechanism remains unclear. Here we show that the network is regulated by a ubiquitin signaling cascade initiated by RNF8 and its partner, UBC13, and mediated by FAAP20, a component of the FA core complex. FAAP20 preferentially binds the ubiquitin product of RNF8-UBC13, and this ubiquitin-binding activity and RNF8-UBC13 are both required for recruitment of FAAP20 to ICLs. Both RNF8 and FAAP20 are required for recruitment of FA core complex and FANCD2 to ICLs, whereas RNF168 can modulate efficiency of the recruitment. RNF8 and FAAP20 are needed for efficient FANCD2 monoubiquitination, a key step of the FA network; RNF8 and the FA core complex work in the same pathway to promote cellular resistance to ICLs. Thus, the RNF8-FAAP20 ubiquitin cascade is critical for recruiting FA core complex to ICLs and for normal function of the FA network.

Pubmed ID: 22705371


  • Yan Z
  • Guo R
  • Paramasivam M
  • Shen W
  • Ling C
  • Fox D
  • Wang Y
  • Oostra AB
  • Kuehl J
  • Lee DY
  • Takata M
  • Hoatlin ME
  • Schindler D
  • Joenje H
  • de Winter JP
  • Li L
  • Seidman MM
  • Wang W


Molecular cell

Publication Data

July 13, 2012

Associated Grants

  • Agency: NIA NIH HHS, Id: AG000688-07
  • Agency: Intramural NIH HHS, Id: Z01 AG000688-07

Mesh Terms

  • Amino Acid Sequence
  • Animals
  • Cell Line, Tumor
  • DNA Repair
  • DNA-Binding Proteins
  • Fanconi Anemia
  • Fanconi Anemia Complementation Group D2 Protein
  • Fanconi Anemia Complementation Group Proteins
  • Green Fluorescent Proteins
  • HEK293 Cells
  • HeLa Cells
  • Humans
  • Immunoblotting
  • Lysine
  • Microscopy, Fluorescence
  • Molecular Sequence Data
  • Mutation
  • Protein Binding
  • Protein Structure, Tertiary
  • RNA Interference
  • Sequence Homology, Amino Acid
  • Signal Transduction
  • Ubiquitin
  • Ubiquitin-Protein Ligases
  • Ubiquitination