Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Rrp6p controls mRNA poly(A) tail length and its decoration with poly(A) binding proteins.

Molecular cell | Jul 27, 2012

http://www.ncbi.nlm.nih.gov/pubmed/22683267

Poly(A) (pA) tail binding proteins (PABPs) control mRNA polyadenylation, stability, and translation. In a purified system, S. cerevisiae PABPs, Pab1p and Nab2p, are individually sufficient to provide normal pA tail length. However, it is unknown how this occurs in more complex environments. Here we find that the nuclear exosome subunit Rrp6p counteracts the in vitro and in vivo extension of mature pA tails by the noncanonical pA polymerase Trf4p. Moreover, PABP loading onto nascent pA tails is controlled by Rrp6p; while Pab1p is the major PABP, Nab2p only associates in the absence of Rrp6p. This is because Rrp6p can interact with Nab2p and displace it from pA tails, potentially leading to RNA turnover, as evidenced for certain pre-mRNAs. We suggest that a nuclear mRNP surveillance step involves targeting of Rrp6p by Nab2p-bound pA-tailed RNPs and that pre-mRNA abundance is regulated at this level.

Pubmed ID: 22683267 RIS Download

Mesh terms: Base Sequence | Cell Nucleus | Cytoplasm | DNA-Directed DNA Polymerase | Exoribonucleases | Exosome Multienzyme Ribonuclease Complex | Exosomes | Gene Expression Regulation, Fungal | Models, Biological | Molecular Sequence Data | Nucleocytoplasmic Transport Proteins | Oligonucleotide Array Sequence Analysis | Poly A | Poly(A)-Binding Proteins | Protein Binding | RNA | RNA, Messenger | RNA-Binding Proteins | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIGMS NIH HHS, Id: GM041752
  • Agency: NIGMS NIH HHS, Id: GM068717
  • Agency: NIGMS NIH HHS, Id: R01 GM041752

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.