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Phosphorylation of distinct sites in MeCP2 modifies cofactor associations and the dynamics of transcriptional regulation.

Mutations in the gene encoding methyl-CpG-binding protein 2 (MeCP2) lead to disrupted neuronal function and can cause the neurodevelopmental disorder Rett syndrome. MeCP2 is a transcriptional regulator that binds to methylated DNA and is most abundant in neuronal nuclei. The mechanisms by which MeCP2 regulates gene expression remain ambiguous, as it has been reported to function as a transcriptional silencer or activator and to execute these activities through both gene-specific and genome-wide mechanisms. We hypothesized that posttranslational modifications of MeCP2 may be important for reconciling these apparently contradictory functions. Our results demonstrate that MeCP2 contains multiple posttranslational modifications, including phosphorylation, acetylation, and ubiquitylation. Phosphorylation of MeCP2 at S229 or S80 influenced selective in vivo interactions with the chromatin factors HP1 and SMC3 and the cofactors Sin3A and YB-1. pS229 MeCP2 was specifically enriched at the RET promoter, and phosphorylation of MeCP2 was necessary for differentiation-induced activation and repression of the MeCP2 target genes RET and EGR2. These results demonstrate that phosphorylation is one of several factors that are important for interpreting the complexities of MeCP2 transcriptional modulation.

Pubmed ID: 22615490

Authors

  • Gonzales ML
  • Adams S
  • Dunaway KW
  • LaSalle JM

Journal

Molecular and cellular biology

Publication Data

July 29, 2012

Associated Grants

  • Agency: NICHD NIH HHS, Id: 2R01HD041462
  • Agency: NICHD NIH HHS, Id: R01 HD041462
  • Agency: NINDS NIH HHS, Id: R01 NS081913
  • Agency: NIEHS NIH HHS, Id: T32 ES007059

Mesh Terms

  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Brain
  • Cell Differentiation
  • Cell Line
  • Cell Nucleus
  • Ether-A-Go-Go Potassium Channels
  • Humans
  • Male
  • Methyl-CpG-Binding Protein 2
  • Mice
  • Molecular Sequence Data
  • Neurogenesis
  • Neurons
  • Phosphorylation
  • Promoter Regions, Genetic
  • Protein Processing, Post-Translational
  • Proto-Oncogene Proteins c-ret
  • Recombinant Fusion Proteins
  • Rett Syndrome
  • Tetradecanoylphorbol Acetate
  • Transcription, Genetic