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Endoglin regulates PI3-kinase/Akt trafficking and signaling to alter endothelial capillary stability during angiogenesis.

Endoglin (CD105) is an endothelial-specific transforming growth factor β (TGF-β) coreceptor essential for angiogenesis and vascular homeostasis. Although endoglin dysfunction contributes to numerous vascular conditions, the mechanism of endoglin action remains poorly understood. Here we report a novel mechanism in which endoglin and Gα-interacting protein C-terminus-interacting protein (GIPC)-mediated trafficking of phosphatidylinositol 3-kinase (PI3K) regulates endothelial signaling and function. We demonstrate that endoglin interacts with the PI3K subunits p110α and p85 via GIPC to recruit and activate PI3K and Akt at the cell membrane. Opposing ligand-induced effects are observed in which TGF-β1 attenuates, whereas bone morphogenetic protein-9 enhances, endoglin/GIPC-mediated membrane scaffolding of PI3K and Akt to alter endothelial capillary tube stability in vitro. Moreover, we employ the first transgenic zebrafish model for endoglin to demonstrate that GIPC is a critical component of endoglin function during developmental angiogenesis in vivo. These studies define a novel non-Smad function for endoglin and GIPC in regulating endothelial cell function during angiogenesis.

Pubmed ID: 22593212


  • Lee NY
  • Golzio C
  • Gatza CE
  • Sharma A
  • Katsanis N
  • Blobe GC


Molecular biology of the cell

Publication Data

July 29, 2012

Associated Grants

  • Agency: NCI NIH HHS, Id: F32CA124139
  • Agency: NCI NIH HHS, Id: F32CA1361252
  • Agency: NHLBI NIH HHS, Id: K99HL103791
  • Agency: NHLBI NIH HHS, Id: R00 HL103791
  • Agency: NCI NIH HHS, Id: R01CA105255

Mesh Terms

  • Adaptor Proteins, Signal Transducing
  • Animals
  • Antigens, CD
  • Capillaries
  • Carrier Proteins
  • Cell Culture Techniques
  • Cell Membrane
  • Cells, Cultured
  • Enzyme Activation
  • Growth Differentiation Factors
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Mice
  • Morphogenesis
  • Neovascularization, Physiologic
  • Neuropeptides
  • Phosphatidylinositol 3-Kinases
  • Protein Transport
  • Proto-Oncogene Proteins c-akt
  • Receptors, Cell Surface
  • Signal Transduction
  • Transforming Growth Factor beta1
  • Zebrafish