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M-Track: detecting short-lived protein-protein interactions in vivo.

We developed a protein-proximity assay in yeast based on fusing a histone lysine methyltransferase onto a bait and its substrate onto a prey. Upon binding, the prey is stably methylated and detected by methylation-specific antibodies. We applied this approach to detect varying interaction affinities among proteins in a mitogen-activated protein kinase pathway and to detect short-lived interactions between protein phosphatase 2A and its substrates that have so far escaped direct detection.

Pubmed ID: 22581371


  • Zuzuarregui A
  • Kupka T
  • Bhatt B
  • Dohnal I
  • Mudrak I
  • Friedmann C
  • Schüchner S
  • Frohner IE
  • Ammerer G
  • Ogris E


Nature methods

Publication Data

June 9, 2012

Associated Grants

  • Agency: Austrian Science Fund FWF, Id: P 21712-B20

Mesh Terms

  • Cell Cycle Proteins
  • Histone-Lysine N-Methyltransferase
  • Humans
  • Methylation
  • Mitogen-Activated Protein Kinases
  • Protein Interaction Mapping
  • Protein Phosphatase 2
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Substrate Specificity
  • TOR Serine-Threonine Kinases
  • Tacrolimus Binding Proteins