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M-Track: detecting short-lived protein-protein interactions in vivo.

Nature methods | Jun 9, 2012

http://www.ncbi.nlm.nih.gov/pubmed/22581371

We developed a protein-proximity assay in yeast based on fusing a histone lysine methyltransferase onto a bait and its substrate onto a prey. Upon binding, the prey is stably methylated and detected by methylation-specific antibodies. We applied this approach to detect varying interaction affinities among proteins in a mitogen-activated protein kinase pathway and to detect short-lived interactions between protein phosphatase 2A and its substrates that have so far escaped direct detection.

Pubmed ID: 22581371 RIS Download

Mesh terms: Cell Cycle Proteins | Histone-Lysine N-Methyltransferase | Humans | Methylation | Mitogen-Activated Protein Kinases | Protein Interaction Mapping | Protein Phosphatase 2 | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Substrate Specificity | TOR Serine-Threonine Kinases | Tacrolimus Binding Proteins

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Associated grants

  • Agency: Austrian Science Fund FWF, Id: P 21712

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