Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Exploring the yeast acetylome using functional genomics.

Cell | May 11, 2012

http://www.ncbi.nlm.nih.gov/pubmed/22579291

Lysine acetylation is a dynamic posttranslational modification with a well-defined role in regulating histones. The impact of acetylation on other cellular functions remains relatively uncharacterized. We explored the budding yeast acetylome with a functional genomics approach, assessing the effects of gene overexpression in the absence of lysine deacetylases (KDACs). We generated a network of 463 synthetic dosage lethal (SDL) interactions involving class I and II KDACs, revealing many cellular pathways regulated by different KDACs. A biochemical survey of genes interacting with the KDAC RPD3 identified 72 proteins acetylated in vivo. In-depth analysis of one of these proteins, Swi4, revealed a role for acetylation in G1-specific gene expression. Acetylation of Swi4 regulates interaction with its partner Swi6, both components of the SBF transcription factor. This study expands our view of the yeast acetylome, demonstrates the utility of functional genomic screens for exploring enzymatic pathways, and provides functional information that can be mined for future studies.

Pubmed ID: 22579291 RIS Download

Mesh terms: Acetylation | Amino Acid Sequence | DNA-Binding Proteins | Gene Deletion | Gene Expression Regulation, Fungal | Genomics | Histone Deacetylases | Histones | Molecular Sequence Data | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Transcription Factors

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: Canadian Institutes of Health Research, Id: MOP-102629
  • Agency: Canadian Institutes of Health Research, Id: MOP-11206
  • Agency: Canadian Institutes of Health Research, Id: MOP97939
  • Agency: NHGRI NIH HHS, Id: R01 HG005853
  • Agency: NHGRI NIH HHS, Id: R01HG005853-01

BioGRID (Data, Interactions)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.