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A resource for the conditional ablation of microRNAs in the mouse.

The importance of miRNAs during development and disease processes is well established. However, most studies have been done in cells or with patient tissues, and therefore the physiological roles of miRNAs are not well understood. To unravel in vivo functions of miRNAs, we have generated conditional, reporter-tagged knockout-first mice for numerous evolutionarily conserved miRNAs. Here, we report the generation of 162 miRNA targeting vectors, 64 targeted ES cell lines, and 46 germline-transmitted miRNA knockout mice. In vivo lacZ reporter analysis in 18 lines revealed highly tissue-specific expression patterns and their miRNA expression profiling matched closely with published expression data. Most miRNA knockout mice tested were viable, supporting a mechanism by which miRNAs act redundantly with other miRNAs or other pathways. These data and collection of resources will be of value for the in vivo dissection of miRNA functions in mouse models.

Pubmed ID: 22570807


  • Park CY
  • Jeker LT
  • Carver-Moore K
  • Oh A
  • Liu HJ
  • Cameron R
  • Richards H
  • Li Z
  • Adler D
  • Yoshinaga Y
  • Martinez M
  • Nefadov M
  • Abbas AK
  • Weiss A
  • Lanier LL
  • de Jong PJ
  • Bluestone JA
  • Srivastava D
  • McManus MT


Cell reports

Publication Data

April 19, 2012

Associated Grants

  • Agency: NHLBI NIH HHS, Id: 5R24HL092851
  • Agency: NIDDK NIH HHS, Id: P30 DK063720
  • Agency: NHLBI NIH HHS, Id: R24 HL092851
  • Agency: NHLBI NIH HHS, Id: R24 HL092851-01

Mesh Terms

  • Animals
  • Embryonic Stem Cells
  • Gene Expression Profiling
  • Genes, Reporter
  • Genotype
  • Mice
  • Mice, Knockout
  • Mice, Transgenic
  • MicroRNAs
  • Models, Animal
  • Models, Genetic