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Laser-capture microdissection and transcriptional profiling of the dorsomedial nucleus of the hypothalamus.

Identifying neuronal molecular markers with restricted patterns of expression is a crucial step in dissecting the numerous pathways and functions of the brain. While the dorsomedial nucleus of the hypothalamus (DMH) has been implicated in a host of physiological processes, current functional studies have been limited by the lack of molecular markers specific for DMH. Identification of such markers would facilitate the development of mouse models with DMH-specific genetic manipulations. Here we used a combination of laser-capture microdissection (LCM) and gene expression profiling to identify genes that are highly expressed within the DMH relative to adjacent hypothalamic regions. Six of the most highly expressed of these genes, Gpr50, 4930511J11Rik, Pcsk5, Grp, Sulf1, and RorĪ², were further characterized by real-time polymerase chain reaction (PCR) analysis and in situ hybridization histochemistry. The genes identified in this article will provide the basis for future gene-targeted approaches for studying DMH function.

Pubmed ID: 22473294


  • Lee S
  • Bookout AL
  • Lee CE
  • Gautron L
  • Harper MJ
  • Elias CF
  • Lowell BB
  • Elmquist JK


The Journal of comparative neurology

Publication Data

November 1, 2012

Associated Grants

  • Agency: NIDDK NIH HHS, Id: P01 DK088761
  • Agency: NIDDK NIH HHS, Id: PL1 DK081182
  • Agency: NIDDK NIH HHS, Id: R01 DK53301
  • Agency: NICHD NIH HHS, Id: R01 HD061539
  • Agency: NIDDK NIH HHS, Id: R37 DK053301
  • Agency: NIDDK NIH HHS, Id: RL1 DK081185
  • Agency: NIDDK NIH HHS, Id: RL1DK081185
  • Agency: NCRR NIH HHS, Id: UL1 RR024923

Mesh Terms

  • Animals
  • Dorsomedial Hypothalamic Nucleus
  • Gene Expression Profiling
  • Immunohistochemistry
  • In Situ Hybridization
  • Laser Capture Microdissection
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Oligonucleotide Array Sequence Analysis
  • RNA, Messenger
  • Real-Time Polymerase Chain Reaction
  • Transcription, Genetic