Preparing your results

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Epigenetic modulation of Homer1a transcription regulation in amygdala and hippocampus with pavlovian fear conditioning.

The consolidation of conditioned fear involves upregulation of genes necessary for long-term memory formation. An important question remains as to whether this results in part from epigenetic regulation and chromatin modulation. We examined whether Homer1a, which is required for memory formation, is necessary for Pavlovian cued fear conditioning, whether it is downstream of BDNF-TrkB activation, and whether this pathway utilizes histone modifications for activity-dependent transcriptional regulation. We initially found that Homer1a knock-out mice exhibited deficits in cued fear conditioning (5 tone-shock presentations with 70 dB, 6 kHz tones and 0.5 s, 0.6 mA footshocks). We then demonstrated that: (1) Homer1a mRNA increases after fear conditioning in vivo within both amygdala and hippocampus of wild-type mice; (2) it increases after BDNF application to primary hippocampal and amygdala cultures in vitro; and (3) these increases are dependent on transcription and MAPK signaling. Furthermore, using chromatin immunoprecipitation we found that both in vitro and in vivo manipulations result in decreases in Homer1 promoter H3K9 methylation in amygdala cells but increases in Homer1 promoter H3 acetylation in hippocampal cells. However, no changes were observed in H4 acetylation or H3K27 dimethylation. Inhibition of histone deacetylation by sodium butyrate enhanced contextual but not cued fear conditioning and enhanced Homer1 H3 acetylation in the hippocampus. These data provide evidence for dynamic epigenetic regulation of Homer1a following BDNF-induced plasticity and during a BDNF-dependent learning process. Furthermore, upregulation of this gene may be regulated through distinct epigenetic modifications in the hippocampus and amygdala.

Pubmed ID: 22457511


  • Mahan AL
  • Mou L
  • Shah N
  • Hu JH
  • Worley PF
  • Ressler KJ


The Journal of neuroscience : the official journal of the Society for Neuroscience

Publication Data

March 28, 2012

Associated Grants

  • Agency: NIDA NIH HHS, Id: DA019624
  • Agency: NIMH NIH HHS, Id: K01 MH069884
  • Agency: NIMH NIH HHS, Id: K01 MH069884-04
  • Agency: NINDS NIH HHS, Id: P30 NS055077
  • Agency: NIDA NIH HHS, Id: R01 DA019624
  • Agency: NIDA NIH HHS, Id: R01 DA019624-04
  • Agency: NIDA NIH HHS, Id: R01 DA019624-05
  • Agency: NIMH NIH HHS, Id: R01 MH096764
  • Agency: NIMH NIH HHS, Id: R01 MH096764-01

Mesh Terms

  • Amygdala
  • Animals
  • Brain-Derived Neurotrophic Factor
  • Carrier Proteins
  • Conditioning, Classical
  • Epigenesis, Genetic
  • Fear
  • Gene Expression Regulation
  • Hippocampus
  • Histone Deacetylase Inhibitors
  • Histone Deacetylases
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mitogen-Activated Protein Kinases
  • Primary Cell Culture
  • Promoter Regions, Genetic
  • Signal Transduction
  • Transcription, Genetic