Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

A link between mitotic entry and membrane growth suggests a novel model for cell size control.

http://www.ncbi.nlm.nih.gov/pubmed/22451696

Addition of new membrane to the cell surface by membrane trafficking is necessary for cell growth. In this paper, we report that blocking membrane traffic causes a mitotic checkpoint arrest via Wee1-dependent inhibitory phosphorylation of Cdk1. Checkpoint signals are relayed by the Rho1 GTPase, protein kinase C (Pkc1), and a specific form of protein phosphatase 2A (PP2A(Cdc55)). Signaling via this pathway is dependent on membrane traffic and appears to increase gradually during polar bud growth. We hypothesize that delivery of vesicles to the site of bud growth generates a signal that is proportional to the extent of polarized membrane growth and that the strength of the signal is read by downstream components to determine when sufficient growth has occurred for initiation of mitosis. Growth-dependent signaling could explain how membrane growth is integrated with cell cycle progression. It could also control both cell size and morphogenesis, thereby reconciling divergent models for mitotic checkpoint function.

Pubmed ID: 22451696 RIS Download

Mesh terms: Adaptor Proteins, Signal Transducing | Cell Cycle | Cell Cycle Proteins | Cell Membrane | Cell Size | Mitosis | Models, Biological | Phosphorylation | Protein Kinase C | Protein Tyrosine Phosphatases | Protein-Tyrosine Kinases | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Signal Transduction | ras-GRF1

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIGMS NIH HHS, Id: F32GM087103-02
  • Agency: NIGMS NIH HHS, Id: GM069602

BioGRID (Data, Interactions)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.