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A toolbox of Cre-dependent optogenetic transgenic mice for light-induced activation and silencing.

Nature neuroscience | May 26, 2012

Cell type-specific expression of optogenetic molecules allows temporally precise manipulation of targeted neuronal activity. Here we present a toolbox of four knock-in mouse lines engineered for strong, Cre-dependent expression of channelrhodopsins ChR2-tdTomato and ChR2-EYFP, halorhodopsin eNpHR3.0 and archaerhodopsin Arch-ER2. All four transgenes mediated Cre-dependent, robust activation or silencing of cortical pyramidal neurons in vitro and in vivo upon light stimulation, with ChR2-EYFP and Arch-ER2 demonstrating light sensitivity approaching that of in utero or virally transduced neurons. We further show specific photoactivation of parvalbumin-positive interneurons in behaving ChR2-EYFP reporter mice. The robust, consistent and inducible nature of our ChR2 mice represents a significant advance over previous lines, and the Arch-ER2 and eNpHR3.0 mice are to our knowledge the first demonstration of successful conditional transgenic optogenetic silencing. When combined with the hundreds of available Cre driver lines, this optimized toolbox of reporter mice will enable widespread investigations of neural circuit function with unprecedented reliability and accuracy.

Pubmed ID: 22446880 RIS Download

Mesh terms: Action Potentials | Animals | Archaeal Proteins | Brain | Electroporation | Halorhodopsins | In Vitro Techniques | Integrases | Light | Luminescent Proteins | Mice | Mice, Transgenic | Neurogenesis | Neurons | Optics and Photonics | Proteins | RNA, Untranslated | Rhodopsin | Wakefulness

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Associated grants

  • Agency: NIDA NIH HHS, Id: DA028298
  • Agency: NIMH NIH HHS, Id: MH085944
  • Agency: NIMH NIH HHS, Id: MH093667
  • Agency: NIMH NIH HHS, Id: MH54671
  • Agency: NIMH NIH HHS, Id: MH90478
  • Agency: NINDS NIH HHS, Id: NS034994
  • Agency: NIDA NIH HHS, Id: R01 DA028298
  • Agency: NIDA NIH HHS, Id: R01 DA028298-02
  • Agency: NHLBI NIH HHS, Id: R01 HL107084
  • Agency: NIMH NIH HHS, Id: R01 MH093667
  • Agency: Howard Hughes Medical Institute, Id:

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