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Tfb6, a previously unidentified subunit of the general transcription factor TFIIH, facilitates dissociation of Ssl2 helicase after transcription initiation.

General transcription factor TFIIH, previously described as a 10-subunit complex, is essential for transcription and DNA repair. An eleventh subunit now identified, termed Tfb6, exhibits 45% sequence similarity to human nuclear mRNA export factor 5. Tfb6 dissociates from TFIIH as a heterodimer with the Ssl2 subunit, a DNA helicase that drives promoter melting for the initiation of transcription. Tfb6 does not, however, dissociate Ssl2 from TFIIH in the context of a fully assembled transcription preinitiation complex. Our findings suggest a dynamic state of Ssl2, allowing its engagement in multiple cellular processes.

Pubmed ID: 22411836

Authors

  • Murakami K
  • Gibbons BJ
  • Davis RE
  • Nagai S
  • Liu X
  • Robinson PJ
  • Wu T
  • Kaplan CD
  • Kornberg RD

Journal

Proceedings of the National Academy of Sciences of the United States of America

Publication Data

March 27, 2012

Associated Grants

  • Agency: NIAID NIH HHS, Id: AI21144
  • Agency: NIGMS NIH HHS, Id: GM36659
  • Agency: NIAID NIH HHS, Id: R01 AI021144
  • Agency: NIGMS NIH HHS, Id: R01 GM036659
  • Agency: NIGMS NIH HHS, Id: R01 GM097260

Mesh Terms

  • Chromatography, Liquid
  • DNA Helicases
  • Gene Deletion
  • Gene Expression Regulation, Fungal
  • Humans
  • Mass Spectrometry
  • Phenotype
  • Phosphorylation
  • Protein Binding
  • Protein Subunits
  • RNA, Messenger
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Temperature
  • Transcription Factor TFIIH
  • Transcription Factors
  • Transcription, Genetic
  • Ultraviolet Rays