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Incomplete splicing, cell division defects, and hematopoietic blockage in dhx8 mutant zebrafish.

Developmental dynamics : an official publication of the American Association of Anatomists | 2012

Vertebrate hematopoiesis is a complex developmental process that is controlled by genes in diverse pathways. To identify novel genes involved in early hematopoiesis, we conducted an ENU (N-ethyl-N-nitrosourea) mutagenesis screen in zebrafish. The mummy (mmy) line was investigated because of its multiple hematopoietic defects.

Pubmed ID: 22411201 RIS Download

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Associated grants

  • Agency: Intramural NIH HHS, United States
    Id: Z01 HG000102-11
  • Agency: Intramural NIH HHS, United States
    Id: ZIA HG000102-13
  • Agency: Intramural NIH HHS, United States
    Id: ZIA HG000102-14
  • Agency: Intramural NIH HHS, United States
    Id: ZIA HG000102-15

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Sequencher (tool)

RRID:SCR_001528

Software for Next-Generation DNA sequencing, Sanger DNA analysis, and RNA sequencing. It contains sequence analysis tools which include reference-guided alignments, de novo assembly, variant calling, and SNP analyses. It has integrated the Cufflinks suite for in-depth transcript analysis and differential gene expression of RNA-Seq data.

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Zebrafish Information Network (ZFIN) (tool)

RRID:SCR_002560

Model organism database that serves as central repository and web-based resource for zebrafish genetic, genomic, phenotypic and developmental data. Data represented are derived from three primary sources: curation of zebrafish publications, individual research laboratories and collaborations with bioinformatics organizations. Data formats include text, images and graphical representations.Serves as primary community database resource for laboratory use of zebrafish. Developed and supports integrated zebrafish genetic, genomic, developmental and physiological information and link this information extensively to corresponding data in other model organism and human databases.

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