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Akt is negatively regulated by the MULAN E3 ligase.

The serine/threonine kinase Akt functions in multiple cellular processes, including cell survival and tumor development. Studies of the mechanisms that negatively regulate Akt have focused on dephosphorylation-mediated inactivation. In this study, we identified a negative regulator of Akt, MULAN, which possesses both a RING finger domain and E3 ubiquitin ligase activity. Akt was found to directly interact with MULAN and to be ubiquitinated by MULAN in vitro and in vivo. Other molecular assays demonstrated that phosphorylated Akt is a substantive target for both interaction with MULAN and ubiquitination by MULAN. The results of the functional studies suggest that the degradation of Akt by MULAN suppresses cell proliferation and viability. These data provide insight into the Akt ubiquitination signaling network.

Pubmed ID: 22410793

Authors

  • Bae S
  • Kim SY
  • Jung JH
  • Yoon Y
  • Cha HJ
  • Lee H
  • Kim K
  • Kim J
  • An IS
  • Kim J
  • Um HD
  • Park IC
  • Lee SJ
  • Nam SY
  • Jin YW
  • Lee JH
  • An S

Journal

Cell research

Publication Data

May 3, 2012

Associated Grants

None

Mesh Terms

  • Cell Proliferation
  • Cell Survival
  • HEK293 Cells
  • HeLa Cells
  • Humans
  • Phosphorylation
  • Proto-Oncogene Proteins c-akt
  • RNA Interference
  • RNA, Small Interfering
  • Recombinant Fusion Proteins
  • Signal Transduction
  • Ubiquitin-Protein Ligases
  • Ubiquitination