• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Identification of a cis-acting element that localizes mRNA to synapses.

Messenger RNA (mRNA) localization and regulated translation can spatially restrict gene expression to each of the thousands of synaptic compartments formed by a single neuron. Although cis-acting RNA elements have been shown to direct localization of mRNAs from the soma into neuronal processes, less is known about signals that target transcripts specifically to synapses. In Aplysia sensory-motor neuronal cultures, synapse formation rapidly redistributes the mRNA encoding the peptide neurotransmitter sensorin from neuritic shafts into synapses. We find that the export of sensorin mRNA from soma to neurite and the localization to synapse are controlled by distinct signals. The 3' UTR is sufficient for export into distal neurites, whereas the 5' UTR is required for concentration of reporter mRNA at synapses. We have identified a 66-nt element in the 5' UTR of sensorin that is necessary and sufficient for synaptic mRNA localization. Mutational and chemical probing analyses are consistent with a role for secondary structure in this process.

Pubmed ID: 22383561

Authors

  • Meer EJ
  • Wang DO
  • Kim S
  • Barr I
  • Guo F
  • Martin KC

Journal

Proceedings of the National Academy of Sciences of the United States of America

Publication Data

March 20, 2012

Associated Grants

  • Agency: NINDS NIH HHS, Id: 5T32NS007449
  • Agency: NINDS NIH HHS, Id: R01 NS045324
  • Agency: NINDS NIH HHS, Id: R01NS045324

Mesh Terms

  • 5' Untranslated Regions
  • Animals
  • Aplysia
  • DNA Mutational Analysis
  • Electrophysiology
  • Genes, Reporter
  • In Situ Hybridization, Fluorescence
  • Neurons
  • Neuropeptides
  • Neurotransmitter Agents
  • Nucleic Acid Conformation
  • Oligonucleotides, Antisense
  • RNA, Messenger
  • Sequence Analysis, RNA
  • Synapses