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Composite low affinity interactions dictate recognition of the cyclin-dependent kinase inhibitor Sic1 by the SCFCdc4 ubiquitin ligase.

The ubiquitin ligase SCF(Cdc4) (Skp1/Cul1/F-box protein) recognizes its substrate, the cyclin-dependent kinase inhibitor Sic1, in a multisite phosphorylation-dependent manner. Although short diphosphorylated peptides derived from Sic1 can bind to Cdc4 with high affinity, through systematic mutagenesis and quantitative biophysical analysis we show that individually weak, dispersed Sic1 phospho sites engage Cdc4 in a dynamic equilibrium. The affinities of individual phosphoepitopes serve to tune the overall phosphorylation site threshold needed for efficient recognition. Notably, phosphoepitope affinity for Cdc4 is dramatically weakened in the context of full-length Sic1, demonstrating the importance of regional environment on binding interactions. The multisite nature of the Sic1-Cdc4 interaction confers cooperative dependence on kinase activity for Sic1 recognition and ubiquitination under equilibrium reaction conditions. Composite dynamic interactions of low affinity sites may be a general mechanism to establish phosphorylation thresholds in biological responses.

Pubmed ID: 22328159

Authors

  • Tang X
  • Orlicky S
  • Mittag T
  • Csizmok V
  • Pawson T
  • Forman-Kay JD
  • Sicheri F
  • Tyers M

Journal

Proceedings of the National Academy of Sciences of the United States of America

Publication Data

February 28, 2012

Associated Grants

  • Agency: Wellcome Trust, Id: 092076
  • Agency: Canadian Institutes of Health Research, Id:

Mesh Terms

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Cell Cycle Proteins
  • Consensus Sequence
  • Cyclin-Dependent Kinase Inhibitor Proteins
  • F-Box Proteins
  • Hydrogen Bonding
  • Models, Molecular
  • Molecular Sequence Data
  • Phosphorylation
  • Protein Conformation
  • Protein Interaction Mapping
  • Protein Processing, Post-Translational
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Static Electricity
  • Surface Plasmon Resonance
  • Ubiquitin-Protein Ligases