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Zfp423 expression identifies committed preadipocytes and localizes to adipose endothelial and perivascular cells.

Progress has been made in elucidating the cell-surface phenotype of primary adipose progenitors; however, specific functional markers and distinct molecular signatures of fat depot-specific preadipocytes have remained elusive. In this study, we label committed murine adipose progenitors through expression of GFP from the genetic locus for Zfp423, a gene controlling preadipocyte determination. Selection of GFP-expressing fibroblasts from either subcutaneous or visceral adipose-derived stromal vascular cultures isolates stably committed preadipocytes that undergo robust adipogenesis. Immunohistochemistry for Zfp423-driven GFP expression in vivo confirms a perivascular origin of preadipocytes within both white and brown adipose tissues. Interestingly, a small subset of capillary endothelial cells within white and brown fat also express this marker, suggesting a contribution of specialized endothelial cells to the adipose lineage. Zfp423(GFP) mice represent a simple tool for the specific localization and isolation of molecularly defined preadipocytes from distinct adipose tissue depots.

Pubmed ID: 22326224

Authors

  • Gupta RK
  • Mepani RJ
  • Kleiner S
  • Lo JC
  • Khandekar MJ
  • Cohen P
  • Frontini A
  • Bhowmick DC
  • Ye L
  • Cinti S
  • Spiegelman BM

Journal

Cell metabolism

Publication Data

February 8, 2012

Associated Grants

  • Agency: NIDDK NIH HHS, Id: DK31405
  • Agency: NIDDK NIH HHS, Id: K01 DK090120
  • Agency: NIDDK NIH HHS, Id: K01 DK090120-02
  • Agency: NIDDK NIH HHS, Id: P30 DK057521
  • Agency: NIDDK NIH HHS, Id: R37 DK031405
  • Agency: NHLBI NIH HHS, Id: T32 HL007604
  • Agency: NHLBI NIH HHS, Id: T32 HL007604

Mesh Terms

  • Adipocytes
  • Animals
  • Cell Differentiation
  • Cell Lineage
  • DNA-Binding Proteins
  • Endothelial Cells
  • Flow Cytometry
  • Gene Expression Profiling
  • Green Fluorescent Proteins
  • Immunohistochemistry
  • Mice
  • Mice, Transgenic
  • Pericytes
  • Stem Cells
  • Transcription Factors