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Subunit architecture of general transcription factor TFIIH.

Structures of complete 10-subunit yeast TFIIH and of a nested set of subcomplexes, containing 5, 6, and 7 subunits, have been determined by electron microscopy (EM) and 3D reconstruction. Consistency among all the structures establishes the location of the "minimal core" subunits (Ssl1, Tfb1, Tfb2, Tfb4, and Tfb5), and additional densities can be specifically attributed to Rad3, Ssl2, and the TFIIK trimer. These results can be further interpreted by placement of previous X-ray structures into the additional densities to give a preliminary picture of the RNA polymerase II preinitiation complex. In this picture, the key catalytic components of TFIIH, the Ssl2 ATPase/helicase and the Kin28 protein kinase are in proximity to their targets, downstream promoter DNA and the RNA polymerase C-terminal domain.

Pubmed ID: 22308316

Authors

  • Gibbons BJ
  • Brignole EJ
  • Azubel M
  • Murakami K
  • Voss NR
  • Bushnell DA
  • Asturias FJ
  • Kornberg RD

Journal

Proceedings of the National Academy of Sciences of the United States of America

Publication Data

February 7, 2012

Associated Grants

  • Agency: NIAID NIH HHS, Id: AI21144
  • Agency: NIDDK NIH HHS, Id: F32-DK080622
  • Agency: NCRR NIH HHS, Id: P41 RR-01081
  • Agency: NIGMS NIH HHS, Id: R01 GM0670167
  • Agency: NIGMS NIH HHS, Id: R01 GM067167
  • Agency: NCRR NIH HHS, Id: RR017573
  • Agency: NCRR NIH HHS, Id: RR023093
  • Agency: NCI NIH HHS, Id: T32 CA09151

Mesh Terms

  • Calmodulin
  • Electrophoresis, Polyacrylamide Gel
  • Models, Molecular
  • Multiprotein Complexes
  • Protein Subunits
  • Saccharomyces cerevisiae
  • Staining and Labeling
  • Transcription Factor TFIIH