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Mec1p associates with functionally compromised telomeres.

In many organisms, telomere DNA consists of simple sequence repeat tracts that are required to protect the chromosome end. In the yeast Saccharomyces cerevisiae, tract maintenance requires two checkpoint kinases of the ATM family, Tel1p and Mec1p. Previous work has shown that Tel1p is recruited to functional telomeres with shorter repeat tracts to promote telomerase-mediated repeat addition, but the role of Mec1p is unknown. We found that Mec1p telomere association was detected as cells senesced when telomere function was compromised by extreme shortening due to either the loss of telomerase or the double-strand break binding protein Ku. Exonuclease I effects the removal of the 5' telomeric strand, and eliminating it prevented both senescence and Mec1p telomere association. Thus, in contrast to Tel1p, Mec1p associates with short, functionally compromised telomeres.

Pubmed ID: 22289863

Authors

  • Hector RE
  • Ray A
  • Chen BR
  • Shtofman R
  • Berkner KL
  • Runge KW

Journal

Chromosoma

Publication Data

June 25, 2012

Associated Grants

  • Agency: NIA NIH HHS, Id: F32 AG022743
  • Agency: NIA NIH HHS, Id: R01 AG019960
  • Agency: NIGMS NIH HHS, Id: R01 GM050752
  • Agency: NIGMS NIH HHS, Id: R01 GM050752
  • Agency: NICHD NIH HHS, Id: T32 HD007104
  • Agency: NCRR NIH HHS, Id: UL1 RR024989

Mesh Terms

  • Cell Proliferation
  • DNA-Binding Proteins
  • Exodeoxyribonucleases
  • Intracellular Signaling Peptides and Proteins
  • Protein-Serine-Threonine Kinases
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Telomere