Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

APC/C(Cdh1)-mediated degradation of the F-box protein NIPA is regulated by its association with Skp1.

PloS one | Dec 29, 2011

http://www.ncbi.nlm.nih.gov/pubmed/22205987

NIPA (Nuclear Interaction Partner of Alk kinase) is an F-box like protein that targets nuclear Cyclin B1 for degradation. Integrity and therefore activity of the SCF(NIPA) E3 ligase is regulated by cell-cycle-dependent phosphorylation of NIPA, restricting substrate ubiquitination to interphase. Here we show that phosphorylated NIPA is degraded in late mitosis in an APC/C(Cdh1)-dependent manner. Binding of the unphosphorylated form of NIPA to Skp1 interferes with binding to the APC/C-adaptor protein Cdh1 and therefore protects unphosphorylated NIPA from degradation in interphase. Our data thus define a novel mode of regulating APC/C-mediated ubiquitination.

Pubmed ID: 22205987 RIS Download

Mesh terms: Adaptor Proteins, Signal Transducing | Amino Acid Sequence | Anaphase-Promoting Complex-Cyclosome | Animals | Cell Cycle Proteins | F-Box Proteins | HEK293 Cells | HeLa Cells | Humans | Mice | Mitosis | Molecular Sequence Data | NIH 3T3 Cells | Nuclear Proteins | Phosphorylation | Protein Binding | Protein Structure, Tertiary | Proteolysis | S-Phase Kinase-Associated Proteins | Ubiquitin-Protein Ligase Complexes | Ubiquitination

Research resources used in this publication

None found

Research tools detected in this publication

Data used in this publication

None found

Associated grants

None

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.