ART: a next-generation sequencing read simulator.
ART is a set of simulation tools that generate synthetic next-generation sequencing reads. This functionality is essential for testing and benchmarking tools for next-generation sequencing data analysis including read alignment, de novo assembly and genetic variation discovery. ART generates simulated sequencing reads by emulating the sequencing process with built-in, technology-specific read error models and base quality value profiles parameterized empirically in large sequencing datasets. We currently support all three major commercial next-generation sequencing platforms: Roche's 454, Illumina's Solexa and Applied Biosystems' SOLiD. ART also allows the flexibility to use customized read error model parameters and quality profiles. AVAILABILITY: Both source and binary software packages are available at http://www.niehs.nih.gov/research/resources/software/art.
Pubmed ID: 22199392
- Huang W
- Li L
- Myers JR
- Marth GT
Bioinformatics (Oxford, England)
February 15, 2012
- Agency: NIEHS NIH HHS, Id: ES101765
- Agency: NHGRI NIH HHS, Id: HG003698
- Agency: NHGRI NIH HHS, Id: HG004719
- Chromosomes, Human, Pair 17
- Genetic Variation
- High-Throughput Nucleotide Sequencing
- Sequence Analysis, DNA