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Global landscape of HIV-human protein complexes.

Human immunodeficiency virus (HIV) has a small genome and therefore relies heavily on the host cellular machinery to replicate. Identifying which host proteins and complexes come into physical contact with the viral proteins is crucial for a comprehensive understanding of how HIV rewires the host's cellular machinery during the course of infection. Here we report the use of affinity tagging and purification mass spectrometry to determine systematically the physical interactions of all 18 HIV-1 proteins and polyproteins with host proteins in two different human cell lines (HEK293 and Jurkat). Using a quantitative scoring system that we call MiST, we identified with high confidence 497 HIV-human protein-protein interactions involving 435 individual human proteins, with ∼40% of the interactions being identified in both cell types. We found that the host proteins hijacked by HIV, especially those found interacting in both cell types, are highly conserved across primates. We uncovered a number of host complexes targeted by viral proteins, including the finding that HIV protease cleaves eIF3d, a subunit of eukaryotic translation initiation factor 3. This host protein is one of eleven identified in this analysis that act to inhibit HIV replication. This data set facilitates a more comprehensive and detailed understanding of how the host machinery is manipulated during the course of HIV infection.

Pubmed ID: 22190034

Authors

  • Jäger S
  • Cimermancic P
  • Gulbahce N
  • Johnson JR
  • McGovern KE
  • Clarke SC
  • Shales M
  • Mercenne G
  • Pache L
  • Li K
  • Hernandez H
  • Jang GM
  • Roth SL
  • Akiva E
  • Marlett J
  • Stephens M
  • D'Orso I
  • Fernandes J
  • Fahey M
  • Mahon C
  • O'Donoghue AJ
  • Todorovic A
  • Morris JH
  • Maltby DA
  • Alber T
  • Cagney G
  • Bushman FD
  • Young JA
  • Chanda SK
  • Sundquist WI
  • Kortemme T
  • Hernandez RD
  • Craik CS
  • Burlingame A
  • Sali A
  • Frankel AD
  • Krogan NJ

Journal

Nature

Publication Data

January 19, 2012

Associated Grants

  • Agency: NIAID NIH HHS, Id: P01 AI090935
  • Agency: NIAID NIH HHS, Id: P01 AI090935
  • Agency: NIAID NIH HHS, Id: P01 AI090935-02
  • Agency: NIGMS NIH HHS, Id: P01 GM073732-05
  • Agency: NIGMS NIH HHS, Id: P41 GM103481
  • Agency: NCRR NIH HHS, Id: P41 RR001081
  • Agency: NCRR NIH HHS, Id: P41RR001614
  • Agency: NIGMS NIH HHS, Id: P50 GM081879
  • Agency: NIGMS NIH HHS, Id: P50 GM081879-02
  • Agency: NIGMS NIH HHS, Id: P50 GM082250
  • Agency: NIGMS NIH HHS, Id: P50 GM082250
  • Agency: NIGMS NIH HHS, Id: P50 GM082250-05
  • Agency: NIGMS NIH HHS, Id: P50GM081879
  • Agency: NIGMS NIH HHS, Id: P50GM082545
  • Agency: NCRR NIH HHS, Id: U54 RR022220

Mesh Terms

  • Affinity Labels
  • Amino Acid Sequence
  • Conserved Sequence
  • Eukaryotic Initiation Factor-3
  • HEK293 Cells
  • HIV Infections
  • HIV Protease
  • HIV-1
  • Host-Pathogen Interactions
  • Human Immunodeficiency Virus Proteins
  • Humans
  • Immunoprecipitation
  • Jurkat Cells
  • Mass Spectrometry
  • Protein Binding
  • Protein Interaction Mapping
  • Protein Interaction Maps
  • Reproducibility of Results
  • Virus Replication